1,3-Benzenedicarboxamide, 5-amino-N1,N3-bis(2,3-dihydroxypropyl)-, hydrochloride (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: screening test, other

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April-October 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

This study meets the requirements of the OECD Principles of Good Laboratory Practice, Paris, 1992

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: RL 92/69/EEC C.4A

Principles of method if other than guideline:

Method: Other: according to guideline RL 92/69/EEC C.4A (DOC-Die-away test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

421-690-3

EC Name:

-

Cas Number:

203515-86-0

Molecular formula:

C14H21N3O6 . ClH

IUPAC Name:

5-amino-N1,N3-bis(2,3-dihydroxypropyl)benzene-1,3-dicarboxamide hydrochloride

Constituent 2

Reference substance name:

3,5-bis[(2,3-dihydroxypropyl)carbamoyl]aniliniumchloride

IUPAC Name:

3,5-bis[(2,3-dihydroxypropyl)carbamoyl]aniliniumchloride

Details on test material:

- Name of test material (as cited in study report): ABA-HCl
- Molecular formula (if other than submission substance):
- Molecular weight (if other than submission substance):
- Smiles notation (if other than submission substance):
- InChl (if other than submission substance):
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type:
- Physical state: Powder
- Analytical purity: ≥98.5
- Impurities (identity and concentrations):
- Composition of test material, percentage of components:
- Isomers composition:
- Purity test date:
- Lot/batch No.: S-31308
- Expiration date of the lot/batch:
- Radiochemical purity (if radiolabelling):
- Specific activity (if radiolabelling):
- Locations of the label (if radiolabelling):
- Expiration date of radiochemical substance (if radiolabelling):
- Stability under test conditions:
- Storage condition of test material:
- Other:

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

117 d

Initial test substance concentration

Initial conc.:

100 mg/L

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

8 d

% Degradation 2

Parameter:

% degradation (DOC removal)

Value:

10.6

Sampling time:

15 d

% Degradation 3

Parameter:

% degradation (DOC removal)

Value:

25.5

Sampling time:

37 d

% Degradation 4

Parameter:

% degradation (DOC removal)

Value:

37.2

Sampling time:

42 d

% Degradation 5

Parameter:

% degradation (DOC removal)

Value:

88.3

Sampling time:

117 d

Applicant's summary and conclusion

Interpretation of results:

not readily biodegradable

Conclusions:

ABA-HCl is biologically degradable, but only after adaptation.

Executive summary:

The test substance (ABA-HCl) is eliminated to 88% in the test flasks within 117 days. The elimination can be divided in several phases. There is a lag-phase with minor degradation in the first 10-15 days. Afterwards elimination occurs to an extent of about 55% within the next ca. 40days. It follows a lag-phase again of about 40 days with a following degradation phase of about 26 days to reach 88% degradation. There can not be defined a degradation window of 10 days, where most degradation occurs. The toxicity control flask gives the same picture of degradation pattern. Only part of the elimination can be caused by physical or chemical elimination. There is an elimination of the test substance in the abiotic control flask to an extent of 41,5% within 117 days. The abiotic elimination is during the whole test about 50 % of the degradation in the test flask. The same elimination pattern can be seen with the adsorption control-flask. So it can be concluded that the test substance is biologically degradable. Biological degradation is expected to occur as well in natural environment as in active sludge plant. Due to rather slow elimination rate (though presumably at least partially caused by the test method used in this study, e.g. use of mineral medium, low sludge content) treatment in active sludge plant should be done only after biological adaptation of the active sludge, at sufficient biomass density and with sufficient reaction time.