Amines, C12-14-alkyl

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study with acceptable restrictions

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

no data on purity, no analytics

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Amines, tallow alkyl

EC Number:

263-125-1

EC Name:

Amines, tallow alkyl

Cas Number:

61790-33-8

IUPAC Name:

61790-33-8

Constituent 2

Reference substance name:

Amines, tallow alkyl

IUPAC Name:

Amines, tallow alkyl

Details on test material:

- Name of test material (as cited in study report): Genamin TA 100 D (Talgfettamin, dest.)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Municipal sewage treatment plant at Hildesheim
- Prereatment: The activated sludge is maintained in an aerobic condition by aeration for four hours and then homogenized with a mixer. The sludge is filtered and the filtrate is subsequently used to initiate inoculation.
- Colony forming units of the inoculum: ca. 18x10^4 (CFU/mL)
- Colony forming units in the test vessels: ca. 18x10^2 (CFU/mL)
The guideline recommends the range of colony forming units between 10^6 and 10^8 (colony forming units/mL). Chosen was a bacterial density, which in experience quickly adapted considering the biodegradation of the ready biodegradable functional control, which is tested usually in each test series. Also the test duration of 28 days allows sufficient time for adaptation of the microorganisms to the test compounds.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

13 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral nutrient medium according to OECD 301B
- Test temperature: 23-24°C
- pH: Control: 7.5; functional control: 7.3; test substance: 7.4; toxicity control: 7.8
- pH adjusted: no


TEST SYSTEM
- Culturing apparatus: 5 L brown glass bottles filled with 3L test medium
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aeration with CO2 free air
- Measuring equipment: Photometer, Dr. Lange CADAS 100
- Details of trap for CO2 and volatile organics if used: series of 3 gas wash bottles


SAMPLING
- Sampling frequency: day 1, 3, 6, 8, 10, 13, 16, 20, 23, 28, 29
- Sampling method: First wash bottle was removed at sampling date. CO2 was determined by titration subsequent to complete adsorption of the released CO2 in a basic solution (Backtitration of the residual Ba(OH)2 with 0,05 N HCI).


CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Toxicity control: 1
- Reference compound: 1

Reference substance

Reference substance:

other: sodium acetate

Results and discussion

Test performance:

The relation between IC and TC content of the test substance in the mineral medium was not determined at the beginning of the test, because the test substance was not fully soluble in the test medium.

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

18

Sampling time:

6 d

Remarks on result:

other: Replicate 1

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

50

Sampling time:

16 d

Remarks on result:

other: Replicate 1

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

64

Sampling time:

29 d

Remarks on result:

other: Replicate 1

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

58

Sampling time:

29 d

Remarks on result:

other: Replicate 2

Details on results:

In the control group a maximum of 28.1 mg CO2/L was formed after 28 days (quality criteria : < 40 mg C02/L).
In the toxicity control the degradation came to 50 % after 13 days in a 10-day window and reached a maximum of 65 % after 28 days.
In case of the test substance the 10 %-pass level was passed after 4 days adaptation phase. In the 10-day window the test substance reached a mean degradation rate of 46 %. After 28 days a mean degradation rate of 61 % was reached.

BOD5 / COD results

Results with reference substance:

The adaptation phase of the functional control changes after 2 days into the degradation phase (degradation > 10 %). The course of the degradation phase is very rapid and reaches a degradation rate of 63 % already on day 8. After 13 days the C02-production corresponds to a degradation rate of 72 %. The quality criteria degradation > 60 % after 14 days was fulfilled.

Any other information on results incl. tables

The test substance must be regarded to be not readily biodegradable in the 10-day window.

Table: CO₂-production and biodegradability for all determination points in the control, test substance samples and toxicity control samples.

Test day	Control mv	Test substance 13 mg/L			Test substance 13 mg/L			Toxicity control
	mg CO2	mg CO2		degr.	mg CO2		degr.	mg CO2		degr.
		gross	net	%	gross	net	%	gross	net	%
1 3 6 8 10 13 16 20 23 28 29 29	6.1 13.5 23.2 29.4 34.3 41.2 48.6 58.9 66.6 74.4 81.1 84.3	5.4 16.5 44.3 60.3 74.7 91.3 105.8 123.2 130.9 141.6 154.3 157.7	0 3.0 21.1 30.9 40.4 50.1 57.2 64.3 64.3 67.2 73.2 73.4	0 3 18 27 35 44 50 56 56 59 64 64	5.1 20.4 46.5 60.8 72.6 91.2 105.4 118.8 127.3 134.8 147.7 151.3	0 6.9 23.3 31.4 38.3 50.0 56.8 59.9 60.7 60.4 66.6 67.0	0 6 20 27 33 44 50 52 53 53 58 58	5.3 43.0 94.0 128.8 143.3 155.3 170.2 186.5 203.1 214.0 229.6 231.7	0 29.5 70.8 99.4 109.0 114.1 121.6 127.6 136.5 139.6 148.5 147.4	0 13 31 44 48 50 54 56 60 61 65 65

degr. = degradation

mv = mean value

Applicant's summary and conclusion

Interpretation of results:

readily biodegradable

Conclusions:

The pass level of 10 [%] (start of the degradation phase) was reached by the test substance at the 4th day. In the 10-day-window the test substance came to a mean degradation rate of 46 [%]. After 28 days a mean degradation rate of 61 [%] was reached.


In order to check the activity of the study system sodium acetate was used as a functional control. The functional control was degraded to 72 [%] after 13 days and therefore the quality criteria of "degradation > 60 [%] after 14 days" was fulfilled.
In the toxicity control the degradation came to a rate of 50 [%] after 13 days and reached a maximum of 65 [%] after 28 days.
The criteria of the guideline that the degradation in the toxicity control should be > 25 % after 14 days was fulfilled.

Executive summary:

The ready biodegradability in the Modified Sturm Test was determined with a non adapted activated studge for the test substance Genamin TA 100 D over a period of 28 days. The study was made from January 30 to February 28, 1996 according to OECD Guidetine 301 B in the DR.U.NOACK-LABORATORIUM FÜR ANGEWANDTE BIOLOGIE.

The test substance was tested in a concentration of 13 [mg/L] in duplicates, corresponding to a carbon content of 10.4 [mgC/L].

The biological degradation of the test subsance was followed by titrimetric analyses of the quantity of CO2 which was produced by the respiration of bacteriae.

The degradation was finished on day 28, the last titration was made on day 29, after the soluble CO2 was turned out over a period of 24 [h].

The CO2 production was calculated as the percentage of total CO2 that the test substance could have theoretically produced based an carbon composition. Biodegradability is therefore expressed as a percentage ThCO2 (calculated by carbon content) and Was calculated for each titration of CO2.

In order to check the activity of the study System sodium acetate was used as functional control. The functional control was degraded to 72 [%] after 13 days and therefore the quality criteria of "degradation > 60 [%] after 14 days“ was fulfiiled.

In the toxicity control the degradation came to a rate of 50 1%) after 13 days and reached a maximum of 65 [ after 28 days.

The criteria of the guideline that the degradation in the toxicity control should be > 25 [%] after 14 days was fufilled.

The biological degradation of the test substance is shown graphically (see table below) in comparison to the readily degradabte functional control and the toxicity control.

The pass level of 10 [%] (start of the degradation phase) was reached by the test substance at the 4th day. In the 10-day window the test substance came to a mean degradation rate of 46 [%]. After 28 days a mean degradation rate of 61 [%] was reached.

Ready biodegradability of the test substance Genamin TA 100 D

in comparison to the functional control and the toxicity control

	Biodegradation [%]
	days
	6	13	20	28
test substance	19	44	54	61
toxicity control	31	50	56	65
functional control	51	72	77	84