2,4,6-trimethylbenzoyl chloride

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trimethylbenzoic acid

EC Number:

207-553-9

EC Name:

2,4,6-trimethylbenzoic acid

Cas Number:

480-63-7

Molecular formula:

C10H12O2

IUPAC Name:

2,4,6-trimethylbenzoic acid

Details on test material:

- Name of test material (as cited in study report): Trisäure Stücke ber. 100%
- Physical state: solid
- Analytical purity: 100%

Method

Target gene:

his, trp

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Metabolic activation system:

aroclor induced rat liver S9

Test concentrations with justification for top dose:

20µg, 100µg, 500µg, 2500µg, 5000µg

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: DMSO

Controls

Controls 1

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: MNNG(TA100, TA1535), NOPD(TA98), AAC(TA1537), 4-NQO(e.coli)

Remarks:

without S9

Controls 2

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: 2-aminoanthracene

Remarks:

with S9

Details on test system and experimental conditions:

METHOD OF APPLICATION: in medium

DURATION
- Preincubation period: none

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

at and above 500µg/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Trimethylbenzoylchlorid (CAS 938-18-1) rapidly hydrolyses to hydrogen chlorid and Trimethylbenzoic acid (CAS 480-63-7), tested in this study.

Applicant's summary and conclusion

Conclusions:

The test substance was not mutagenic in the Ames test.