2,4,6-trimethylbenzoyl chloride

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

5 mice per sex and dose are injected i.p. with different doses of the test substance. Clinical signs and lethality are evaluated during a 14 day observation period and the LD50 is determined. Surviving animals are necropsied and gross changes recorded.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trimethylbenzoyl chloride

EC Number:

213-339-6

EC Name:

2,4,6-trimethylbenzoyl chloride

Cas Number:

938-18-1

Molecular formula:

C10H11ClO

IUPAC Name:

2,4,6-trimethylbenzoyl chloride

Details on test material:

- Name of test material (as cited in study report): 2.4.6-trimethylbenzoylchlorid
- Physical state: liquid
- Analytical purity: no data

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Herilan, H.Eggersmann KG
- Age at study initiation: no data
- Weight at study initiation: 22-28g
- Fasting period before study: 15-20h
- Acclimation period: no data

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

olive oil

Doses:

200mg/kg
700mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: 15min, 30min, 1h, 2h, 5h after injection, daily thereafter. Weighing performed on days 2-4, 7, 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 700 mg/kg bw

Based on:

test mat.

Mortality:

all animals in the high dose died within7days, all animals in the low dose survived

Clinical signs:

Dypnoe, apathy, bad general state in both groups
tremor, jerks, spastic gait, stretching, piloerection in the high dose

Body weight:

high dose: decrease in males, slight gain in females (until death on days 2-4)
low dose: decrease until day four, recovery to initial weight until day 14

Gross pathology:

dead animals: substance deposits in the peritoneum
necropsied survivors: no findings

Applicant's summary and conclusion

Conclusions:

The LD50 in mice after i.p. injection of the test substance was found to be greater than 200mg/kg, but lower than 700mg/kg.