N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22.07.1997 - 24.07.1997 (experimental period)

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EEC Directive 92/32/EEC, Annex V, Part C

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

EC Number:

229-761-9

EC Name:

N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Cas Number:

6711-48-4

Molecular formula:

C10H25N3

IUPAC Name:

N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Details on test material:

- Name of test material (as cited in study report): Tetramethyldipropylentriamin; roh ber. 100 %
- Physical state: liquid
- Analytical purity: 51.7 %
- Impurities (identity and concentrations): 27.4 % DMAPA (CAS-# 109-55-7), 0.3 % water
- Lot/batch No.: container 314
- Homogeneity: homogeneous
- Stability: unstable in acids
- Storage conditions: room temperature
- Solubility in water: mixable

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 0.78, 12.5 and 100 mg/L were analysed

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in M4 medium for about 10 minutes at appr. 22 ± 1 °C.
The nominal concentration of the stock solution was 100 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this olone was cultured and breaded continuously in the "Labor für Oekotoxikologie" of the BASF AG.
- Age at study initiation (mean and range, SD): 2 - 24 h
- Age of stock animals: 2 - 4 weeks

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 - 3.20 mmol/L

Test temperature:

20.2 - 21.6

pH:

8.0 - 9.8
7.1 - 8.3 (neutralised)

Dissolved oxygen:

7.8 - 8.6 mg/L

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100
12.5 (neutralized), 25 (neutralized), 50 (neutralized), 100 (neutralized)

Analytically determined concentrations (mg/l):
<2 (0 nominal), < 2 (0.78 nominal), 13 (12.5 nominal), 99 (100 nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tubes with flat bottom (20 ml)
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding: none


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Alkalinity up to pH 4.3: 0.80 - 1.00 mmol/L
- Ca/mg ratio: 4:1
- Conductivity: 617 µS/cm
- Total hardness: 2.40 mmol/L

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
- Light intensity: Artificial light, type warm white (eg. OSRAM L58 W31), about 2 - 7 µE/(m²s) at a wave length of 400 - 700 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Test concentrations: 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: not neutralized

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

40.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 32.4 - 50.9 mg/L; not neutralized

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: not neutralized

Results with reference substance (positive control):

The EC50 (24h) of the reference substance was 1.16 mg/L

Any other information on results incl. tables

Summary of the effective concentrations of the test substance which caused 0%, 50%, or 100% immobilization after various periods of exposure

Time (h)	EC0	EC50	CL95%	EC100
3	≥ 100	> 100	-	> 100
6	≥ 100	> 100	-	> 100
24	50.0	73.8	64.8-84.1	> 100
48	12.5	40.6	32.4-50.9	100

 

Number of live (mobile) test animals after various periods of exposure to various concentrations of the test substance

Conc [mg/L]	Total number of mobile test animals
[h]	3h	6h	24h	48h
Control	20	20	19	18
100	20	18	3	0
50	20	20	19	8
25	20	20	20	15
12.5	20	20	20	20
6.25	20	20	20	19
3.13	20	20	20	20
1.56	20	20	20	20
0.78	20	20	20	19
100 neutr	20	20	10	2
50 neutr	20	20	20	15
25 neutr	20	20	20	20
12.5 neutr	20	20	20	20

 

 

Summary of the analysis of the concentrations of the test substance. The values given are the relative minimum and maximum concentrations of the test substance in the test solutions at various times of the study. The values are given in percent of the nominal concentrations.

Time (h)	Stocked with daphnids	Minimum (%)	Maximum (%)
0	No	99.0	104.0
48	No	96.0	128.2
48	yes	96.0	102.6

 

pH and oxygen values

Conc [mg/L]	pH after 0 h*	pH after 48 h**	O2 [mg/L] after 0 h*	O2 [mg/L] after 48 h**
Control	8.0	8.0	8.4	8.2
100	9.8	8.7	8.3	7.9
50	9.4	8.4	8.3	7.8
25	9.1	8.2	8.3	7.9
12.5	9.0	8.2	8.2	7.9
6.25	8.5	8.1	8.5	7.9
3.13	8.3	8.1	8.5	7.9
1.56	8.2	8.1	8.5	8.0
0.78	8.1	8.0	8.6	8.1
100 neutr	7.1	7.6	8.3	8.0
50 neutr	7.3	7.8	8.3	7.9
25 neutr	7.5	7.9	8.3	8.0
12.5 neutr	7.6	8.0	8.3	7.9

* measured in the additional replicate 5 of each concentration

** measured in replicate 1 of each concentration