N-methoxy-1-(2,4,6-trichlorophenyl)propan-2-amine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-04-17 until 2015-07-24

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in accordance with current guidelines and GLP compliance.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Name of test material (as cited in study report): CA5204A (MOAT)
- Physical state: Brown liquid
- Analytical purity: 93.2 %
- Lot/batch No.: SMU2DP12003
- Recertification date of the lot/batch: End of September 2015
- ThOD: 1.444 g O2/g considering total nitrification; 1.222 g O2/g considering no nitrification

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage treatment plant
Ruhrverband Kläranlage, Sunthelle 6, 57392 Schmallenberg, Germany
- Date of sampling: 28th April 2015
- Storage conditions: No storage
- Preparation of inoculum for exposure: No washing, the concentration was adjusted to 3.0 g/L and verified by dry mass
measurement.
- Pretreatment: Kept aerobic until use
- Concentration of sludge in the test: 29.6 mg dry mass/litre

Duration of test (contact time):

28 d

Initial conc.:

44 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

(63.5 mg ThOD considering total nitrification and 53.7 mg ThOD considering the total lack of nitrification.)

Details on study design:

- The concentration in the test assays were 44 mg per litre mineral test medium (11 mg/250 mL). 44 mg test item correspond to 63.5 mg ThOD considering total nitrification and 53.7 mg ThOD considering the total lack of nitrification. The required amount of test item was added directly on a weight basis via Teflon discs. Subsequently, the required volume of aqueous reference stock solution and mineral medium were added to the vessels.
- The concentration in the test vessels with reference item (procedural control, toxicity control) was 100 mg per litre mineral test
medium (25 mg/250 mL).
- A further solution containing both test and reference item at the same concentrations as in the individual solutions (test item 44 mg/L, reference item 100 mg/L) was prepared to determine the possible toxicity of the test item against the inoculum.
- Further flasks with mineral medium only were prepared for inoculum controls.
- Abiotic controls were also prepared to measure any possible abiotic degradation; a solution of the test item at about 44 mg/L was sterilized by the addition of 1 mL/L HgCl2 (10 g/L).
- In all test assays except of the abiotic controls, 2.49 mL of the inoculum stock solution were transferred for inoculation (per 250 mL) resulting in a concentration of 29.6 mg dry mass/litre (7.40 mg dry mass/250 mL).

Reference substance:

benzoic acid, sodium salt

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Results with reference substance:

The reference item was degraded by an average of 89 % by Exposure Day 14, thus confirming suitability of the activated sludge. At the end of the test (Day 28), the reference item was degraded by an average of 102 %.

Results are provided in the attached document.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The biochemical oxygen demand (BOD) of the test item in the test media was in the range of the inoculum controls throughout the
study period of 28 days. Consequently, the test item was not biodegradable under the test conditions within 28 days.

Executive summary:

At the Fraunhofer Institute for Molecular Biology and Applied Ecology the ready biodegradability of the test item was investigated according to the manometric respirometry test design and carried out according to the OECD guideline for Testing of Chemicals No. 301 F (1992) and the Council Regulation (EC) No 440/2008 of 30 May 2008.

The ready biodegradability of the test item was investigated at a concentration of 44 mg/L in a manometric respirometry test using domestic aerobic activated sewage sludge incubated at 22°C in the dark over 28 days under continuous stirring. The biochemical oxygen demand (BOD) was measured daily and the percentage degradation was calculated from the theoretical oxygen demand (ThOD) after correcting for the BOD of inoculum controls.

The biochemical oxygen demand of the test item in the test media was in the same range as the inoculum controls, so the test item exhibited no significant additional oxygen demand. Consequently, the test itemwas not biodegradable under the conditions of the test. The test item had no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration. In the procedural controls, the reference substance sodium benzoate was degraded by an average of 89 % by exposure day 14, and reached an average biodegradation of 102 % by the end of the test (day 28), thus confirming suitability of the activated sludge.

Description of key information

The biochemical oxygen demand (BOD) of the test item in the test media was in the range of the inoculum controls throughout the

study period of 28 days. Consequently, the test item was not biodegradable under the test conditions within 28 days.

Key value for chemical safety assessment

Biodegradation in water:

under test conditions no biodegradation observed

Type of water:

freshwater