Dodecylbenzenesulphonic acid, compound with 2-amino-2-methylpropan-1-ol (1:1)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Remarks:

the tested substance is AMP one of the components of the compound

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: meets generally accepted scientific standards, and acceptable for assessment. The reports presents of 4 tests, two of which are the final replicate experiments.

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

close vessels

Principles of method if other than guideline:

Not applicable

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):Not applicable

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The chemical was added at increasing concentrations from a test solution prepared from a continuously illuminated algae stock culture, prepared with nutrient solution, and was placed in front of a light wall. A single vessel was tested per concentration.

Test organisms (species):

Scenedesmus sp.

Details on test organisms:

No data

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Details on test conditions:

The investigations according to the guideline give false results in the case of volatile substances due to the decreasing concentration. Closing of the test vessel led to growth inhibition or death in the absence of the test material. By previous passage of CO2-enriched air and variation of the air space in the test vessel, the conditions could be optimized for the growth of the algae in the closed vessel during the 96 hour test so that the growth rate was comparable to that in an open vessel. The chemical was added at increasing concentrations from a test solution prepared from a continuously illuminated algae stock culture, prepared with nutrient solution, and was placed in front of a light wall. The test vessels were shaken slightly from time to time in order to prevent sedimentation of the algae.

Reference substance (positive control):

no

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

402 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

540 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

522 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Remarks:

(algae concentration)

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

609 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

cell number

Details on results:

Four tests were performed, two of which are replicate experiments (EC50=522mg/L and EC50=609 mg/L).

Results with reference substance (positive control):

Not applicable

Not applicable

Validity criteria fulfilled:

not specified

Conclusions:

The growth inhibition was assessed in 4 experiments, two of which are replicate experiments (EC50=522mg/L and EC50=609 mg/L).

Executive summary:

The effect on the growth of algae was investigated and the inhibition of microbial-degradation was studied. The substance concentrations found for 50% inhibition show that AMP 90 has a weak one. The values of the bacterial inhibition test were determined from the curves of the individual experiments through an average inhibition curve.

Reference 2

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Remarks:

the tested substance is DDBSA one of the components of the compound

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

The concentration of the test substance in the test solution was analyzed at the beginning (0 hr) and at the end (72 hrs) of the test. About 2 mL sample (3 replicates) was taken from prepared vessels at 0 hours and at 72 hours, firstly 10 mL of test solutions of 3 flasks of each concentration were pooled and the solutions were centrifuged slightly(5000 rpm, 10 min) to remove grown algae. Upper phase of centrifuged solutions were taken and put into the analytical vial and then analyzed.

Vehicle:

yes

Details on test solutions:

Preparation method of test solution :After 103 mg of the test substance was added in a 1,000 mL beaker, and the dilution water (OECD nutrient medium) was added up to the mark of the beaker. This solution was then stirred for about 30 minutes. Serial dilutions of this test solution were prepared with the dilution water to give the desired series of exposure levels which were nominally 50, 25, 12.5 and 6.3 mg/L. For the control group, only the OECD culture medium was used. Test solution became turbid slightly but it was not filtered into consideration of characterization of test substance. This test substance is surfactant (by MSDS) and OECD document “Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (OECD, 2000) recommend that separation techniques should not be applied to surfactant solution. Therefore, the filtration process was not performed for preparation of test solution.

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

Pseudokirchnerella subcapitata - Strain No. : UTEX 1648

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Hardness:

Hardness (as CaCO3): 45.5 mg/L

Test temperature:

23.1 +/- 0.1 deg C

pH:

7.08-8.27

Nominal and measured concentrations:

Test concentrations were as follows :- Nominal : control, 6.3, 12.5, 25.0, 50.0 and 100.0 mg/L- Mean measured : control, 7.9, 15.1, 29.2, 56.1 and 101 mg/L

Details on test conditions:

- Medium : sterile OECD Nutrient medium (OECD TG, 2006)- Light intensity : 81- 89 μE/m2/s- Initial cell density : 5.0 × 103 cells/mL- Test design : 3 replicates

Reference substance (positive control):

no

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

65.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 61.7-69.8

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

21 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 7.9-30.1

Key result

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

7.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

15.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

A 72 h NOEC of 7.9 mg/L has been determined for the effects of the test substance on growth rate of the Pseudokirchnerella subcapitata.

Validity criteria fulfilled:

yes

Conclusions:

The substance is less toxic to algae under the condition of the test.
A 72 h NOEC of 7.9 mg/L has been determined for the effects of the test substance on growth rate of the Pseudokirchnerella subcapitata.

Executive summary:

The study was conducted in accordance with the OECD TG 201 with Pseudokirchneriella subcapitata. It was performed with control and nominal concentrations of 6.3, 12.5, 25.0 and 100 mg/L each in triplicates. Cultures were incubated on a shaking incubator under continuous illumination at 23.1±1°C for 72 hours. Growth was monitored daily by determining the cell density of each culture by direct counts using a microscope. The 72hr-EC₅₀values of this study were 65.4 mg/L (measured) for average growth rate and 21.0 mg/L (measured) for yield respectively. The 72hr-NOEC values were 7.9 mg/L (measured) for average growth rate and yield.

Description of key information

A study on DDBSA was conducted in accordance with the OECD TG 201 withPseudokirchneriella subcapitata. It was performed with control and nominal concentrations of 6.3, 12.5, 25.0 and 100 mg/L each in triplicates. Cultures were incubated on a shaking incubator under continuous illumination at 23.1±1°Cfor 72 hours. Growth was monitored daily by determining the cell density of each culture by direct counts using a microscope. The 72hr-EC50 values of this study were 65.4 mg/L (measured) for average growth rate and 21.0 mg/L (measured) for yield respectively. The 72hr-NOEC values were 7.9 mg/L (measured) for average growth rate and yield.

For AMP the effect on the growth of algae was investigated and the inhibition of microbial-degradation was studied. The growth inhibition was assessed in 4 experiments, two of which are replicate experiments (EC50=522mg/L and EC50=609 mg/L).

Key value for chemical safety assessment

EC50 for freshwater algae:

65.4 mg/L

EC10 or NOEC for freshwater algae:

7.9 mg/L

Additional information

Data on both dissociation products DDBSA and AMP are available. In a worst case approach it is assumed that the toxicity to algae is determined by DDBSA, which leads to a NOEC of 7.9 mg/L.