Dodecylbenzenesulphonic acid, compound with 2-amino-2-methylpropan-1-ol (1:1)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

the tested substance is AMP one of the components of the compound

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: meets generally accepted scientific standards, well-documented, and acceptable for assessment

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians" (USEPA, 1975

Deviations:

no

Remarks:

Not specified in report

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Deionized water was used in the preparation of all stock solutions.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnids (Daphnia magna) first instar < 24 hours old. The daphnia used in the test were cultured at the testing facilities, and the adult daphnia were fed a suspension of trout chow and alfalfa daily until 24 hours prior to testing.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

225 ppm as CaCO3

Test temperature:

19-20C

pH:

8.1 in the control, 8.3 (100mg/L AMP), 8.9 (320 mg/L AMP), and 9.4 (1000 mg/L AMP)

Dissolved oxygen:

Dissolved oxygen levels ranging from 8.3 mg/L in the control at study start, and levels of 8.5 (control), 8.5 (100mg/L AMP), 8.6 (320 mg/L AMP), and 8.6 mg/L O2 (1000 mg/L AMP) at 48 hours.

Nominal and measured concentrations:

Nominal- 0, 100, 180, 320, 560, and 1000 mg/L AMP-95

Details on test conditions:

Daphnias (Daphnia magna) first instar < 24 hours old. Test conditions were a temperature range of 19-20C, and a photoperiod of 16 hours light/ 8 hour dark photcycle. Test solution (200mL) was placed in 250mL beakers, and ten daphnias were added per test solution.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

193 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 100-320 mg/L

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

240 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 212-272 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

There was no additional data provided on the results of the range-finding study referenced in the methods section.

Reported statistics and error estimates:

A computerized program calculated the LC50 for Daphnia based on the Probit dose-response model.

Not applicable

Validity criteria fulfilled:

not specified

Conclusions:

The 48-hour Daphnia magna LC50 was calculated to be 193 mg/L.

Executive summary:

AMP-95 (P-2031, 2-amino-2-methyl-propanol), lot number 216 (AMP-94.31%, Water 5.69%, NVM 0.0004%, Neutral Eq. 94.68), was tested for the acute t toxicity effects on daphnias. The 24 and 48 hr LC50 and "no effect" levels were determined by ABC Laboratories, Inc., Columbia, Missouri. Test Conditions-daphnias (Daphnia magna) first instar ≤24 hr old. Test temperature 19-20C. Photoperiodicity 16 hr day light. 200 ml test solution in 250 ml beakers. Ten daphnias per test solution. The AMP-95 concentrations were tested in duplicate at 0, 100, 180, 320, 560 and 1000 mg/liter (ppm). Results- There was no significant changes in the water quality parameters except an increase in pH at the ≥320 mg/l concentration. The estimated LC50 at 24 hr was 240 (212-272) mg/liter and at 48 hr was 193 (100-320) mg/liter (95 percent confidence limits are in parenthesis). The 48 hr “no effect” level was 100 mg/liter.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Remarks:

the tested substance is DDBSA one of the components of the compound

Adequacy of study:

key study

Study period:

May 8, 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna, <24 h old.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20±1 degree C

pH:

pH 7.8-8.0

Dissolved oxygen:

dissolved oxygen 7.7-8.0;

Nominal and measured concentrations:

0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L

Details on test conditions:

Seven concentrations and a control were tested (0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L). Physicochemical parameters were maintained within acceptable ranges (temperature 20±1 degree C; dissolved oxygen 7.7-8.0; pH 7.8-8.0). At 48 hours, 0/20 daphnids were immobile in the 0.4 through 3.5 mg/L concentrations, 14/20 in 6.0 mg/L, and 20/20 in 10 mg/L. Graphical interpolation was used to calculate the EC50 value.

Reference substance (positive control):

not required

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (95% 3.5-6.0 mg/L)

Details on results:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Executive summary:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Description of key information

For DDBSA a 48 h EC50 of 5.2 mg/L has been determined for the effects on Immobility of the Daphnia magna.

AMP-95 (P-2031, 2-amino-2-methyl-propanol), lot number 216 (AMP-94.31%, Water 5.69%, NVM 0.0004%, Neutral Eq. 94.68), was tested for the acute t toxicity effects on daphnia. The 24 and 48 hr LC50 and "no effect" levels were determined by ABC Laboratories, Inc., Columbia, Missouri. Test Conditions-daphnia (Daphnia magna) first instar ≤24 hr old. Test temperature 19-20C. Photoperiodicity 16 hr day light. 200 ml test solution in 250 ml beakers. Ten daphnia per test solution. The AMP-95 concentrations were tested in duplicate at 0, 100, 180, 320, 560 and 1000 mg/L (ppm). Results- There was no significant changes in the water quality parameters except an increase in pH at the ≥320 mg/L concentration. The estimated LC50 at 24 hr was 240 (212-272) mg/L and at 48 hr was 193 (100-320) mg/L(95 percent confidence limits are in parenthesis). The 48 hr “no effect” level was 100 mgL.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

5.2 mg/L

Additional information

Data on both dissociation products DDBSA and AMP are available. In a worst case approach it is assumed that the toxicity to daphnia is determined by DDBSA, which leads to a LC50 of 5.2 mg/L.