Ethanol, 2-(ethenylthio)-

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1967-09-26/27 and 1967-10-02

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

Please refer to "Principles of method"

Principles of method if other than guideline:

according to BASF-internal standard

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol, 2-(ethenylthio)-

EC Number:

608-554-0

Cas Number:

3090-56-0

Molecular formula:

C4H8OS

IUPAC Name:

Ethanol, 2-(ethenylthio)-

Test material form:

liquid

Specific details on test material used for the study:

No specific details for test material were provided.

Test animals

Species:

rat

Strain:

other: US

Sex:

male/female

Details on test animals or test system and environmental conditions:

No details were provided.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: water emulsion containing Traganth

Details on oral exposure:

- Application form: emulsion
- Concentration in vehicle: 2, 20 and 30 % (v/v)

Doses:

0.2, 1.6, 2.5 and 3.2 mL/kg bw
213.38, 1707.04, 2667.25 and 3414.08 mg/kg bw (calculated from mL/kg with a density of 1.0669 g/cm^3)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

No statistical analysis was performed.

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 3 mL/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 3 200.7 mg/kg bw

Based on:

test mat.

Remarks on result:

other: calculated from mL/kg with a density of 1.0669 g/cm^3

Mortality:

3.2 mL/kg bw: 6 animals died within 24 h after application. 1 animal died between 24 and 48 h after application.
2.5 mL/kg bw: 2 animals died within 48 h after application. 1 animal died between 48 h and 7 days after application.
1.6 mL/kg: 1 animal died between 48 h and 7 days after application.
No further mortality was observed.

Clinical signs:

3.2 mL/kg: ventral/side position, dyspnea, apathy, anesthetic like behavior, slack body tonus, striking blood flow in extremities, ears, nose and lips, secretion from nose. All symptoms lasted 2 days, gradually improved and were not detected in survivors from day 5 onward.
2500 cmm/kg: Belly/side position, difficult breathing and apathy was detected directly after application. After 24 h eyes were clotted and the nose was encrusted with blood. Symptoms lasted 1 day, gradually improved and were not detected in survivors from day 4 onward.
1.6 mL/kg: Staggering walk, increased breathing rate, pilo erection, hunched position, apathy was observed for over 1 day. After 2 days symptoms nearly disappeared.
0.2 cmm/kg: Staggering walk, increased breathing rate and pilo erection was observed for over 1 day. After 2 days symptoms nearly disappeared.

Body weight:

Body weights were only determined before treatment.

Gross pathology:

3.2 mL/kg: 1x blood encrusted nose, 1x chronic bronchitis
2.5 mL/kg: 2x blood encrusted nose, 1x dubiose fatty liver, 1x chronic bronchitis
No other observations were made in survivors or animals that died after treatment.

Other findings:

No other observations were made.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met