Registration Dossier
Registration Dossier
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EC number: 203-661-5 | CAS number: 109-28-4
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Brachydanio rerio were exposed in a semi-static test to a concentration range of 0.1 to 1.05 mg/L of the test item. The calculated LC50 after 96 hours of exposure to the test item was 0.22 (95% CL: 0.19 - 0.26) mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.22 mg/L
Additional information
The test substance was tested in an acute toxicity test with Brachydanio rerio (zebra fish) under semi-static conditions in accordance with EEC method C.1 and OECD Guideline 203. Zebra fish were exposed to 0.1, 0.18, 0.32, 0.58 and 1.05 mg test item/L. No chemical analysis of the test concentrations were performed during the test and all concentrations mentioned are nominal concentrations. The LC50 (96h) was calculated to be 0.22 mg/L with 95% confidence limits of 0.19 and 0.26 mg/L. The highest concentration causing no mortality (no observed effect concentration, NOEC) after 96 hours amounted to 0.10 mg/L, whereas 100% mortality was produced within 30 hours at 0.32 mg/L. At the concentrations of 0.32, 0.58 and 1.05 mg/L the fish showed reduced activity before their death.
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