Isobutyryl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401. A test group consisting of 5 animals/sex was treated by single gavage application with the test substance dissolved in olive oil. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyryl chloride

EC Number:

201-194-1

EC Name:

Isobutyryl chloride

Cas Number:

79-30-1

Molecular formula:

C4H7ClO

IUPAC Name:

2-methylpropanoyl chloride

Details on test material:

- Name of test material (as cited in study report): Isobuttersäurechlorid
- Substance number: 79/625

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Wiga
- Weight at study initiation: male mean weight: 202 g; female mean weight: 172 g
- Fasting period before study: 15-20 hours before administration

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

VEHICLE
Concentration in vehicle: 2.94 - 29.4 % (w/v)

MAXIMUM DOSE VOLUME APPLIED:
5 mL/kg

Doses:

147, 316, 464, 681, 1000, 1470 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: at the start of the test and after 2-4, 7 and 13 days
- Necropsy of survivors performed: yes
- Examinations performed: clinical signs, body weight, gross pathology

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 1 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 680 - < 1 000 mg/kg bw

Based on:

test mat.

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 1 100 mg/kg bw

Based on:

test mat.

Mortality:

No deaths occurred at 147-681 mg/kg bw
5/5 males and 1/5 females died at 1000 mg/kg bw
10/10 animals died at 1470 mg/kg bw
All deaths were observed 1 hour - 1 day after administration of the test substance.

Clinical signs:

other: 147 and 316 mg/kg bw: no clinical signs 464 mg/kg bw: dyspnea, apathy, uncoordinated movement, poor general state 681 mg/kg bw: dyspnea, apathy, uncoordinated movement, bristly fur, poor general state 1000 mg/kg bw: dyspnea, apathy, anomalous lying behavi

Gross pathology:

Animals which died during the test: Hart: atrial dilatation, local acute hyperaemia; Stomach: extensive haemorrhagic ulceration with haematic contents (corrosive gastritis); Bowels: strong reddening of the mucosa with haemorrhagic contents.
Animals sacrificed at the end of the observation period: no abnormalities.