Registration Dossier
Registration Dossier
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EC number: 603-562-0 | CAS number: 13223-43-3
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The test was conducted for 96-hours at 22 to 24 °C, under continuous illumination at 360 to 440 footcandles (3900 to 4700 lux) and a shaking rate of 100 rpm. The dilution water used was Algal Assay Procedure (AAP) medium.
The nominal concentrations used were 6.3, 13, 25, 50 and 100 mg/L which correspond to mean measured concentrations of 5.7, 12, 23, 47 and 92 mg/L.
The 72 hour EC50 for growth rate, biomass and cell density is >92 mg/L (mean measured), this corresponds to a nominal concentration of >100 mg /L. The 72 hour NOEC is 92 mg/L (100 mg/L nominal).
Additional testing to further define the EC50 values was not performed since the highest nominal concentration tested was equivalent to the maximum concentration required by the U.S. EPA and OECD guidelines (100 mg/L).
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 92 mg/L
Additional information
The acute toxicity of the test material and water to the Freshwater Green Alga, Pseudokirchneriella subcapitata was investigated during a study conducted at Springborn Smithers Laboratories. The test was performed from February 20 to 24, 2006 and was conducted according to OECD Guideline 201 (Alga, Growth Inhibition Test). This study is presented as the key information as the reliability rating for this study is 1, according to the criteria of Klimisch, 1997.
Based upon the results of the algae study, the only aquatic study available, the registered substance does not meet the criteria for classification according to Directive 67/548/EEC (DSD) or EC Regulation 1272/2008 (CLP).
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