Registration Dossier
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EC number: 213-947-1 | CAS number: 1068-55-9
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-10-26 to 2013-06-15
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Principles of method if other than guideline:
- The Guidlines for the Testing of Chemicals - State Environmental Protection Administration (SEPA) of China No.301 F
- GLP compliance:
- no
Test material
- Reference substance name:
- Chloro(1-methylethyl)magnesium
- EC Number:
- 213-947-1
- EC Name:
- Chloro(1-methylethyl)magnesium
- Cas Number:
- 1068-55-9
- Molecular formula:
- C3H7ClMg
- IUPAC Name:
- chloro(1-methylethyl)magnesium
- Reference substance name:
- Isopropylmagnesium chloride
- IUPAC Name:
- Isopropylmagnesium chloride
- Test material form:
- other: liquid, 20% in Tetrahydrofuran
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Species: Activated sludge from Xiaohe sewage treatment plant collected 2012-12-07
Pre-conditioning of inoculum:
- supernatant floating on the surface was discarded
- sludge was washed 3 times with 10 L mineral medium
- aeration
- mineral medium was adjusted to pH 7.4
- 30 mg SSL was in solution
- 127.3 mg/L test substance was added - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 127.3 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- other: ThOD
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2.82
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference item Sodium benzoate was sufficiently degraded to a mean of 77.09% after 14 days, and to a mean of 93.07 % after 28 days of incubation, based on ThOD, thus confirming the suitability of the used activated sludge inoculum.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The biodegradation of Isopropyl magnesium was 2.82% after 28 d treatment. Therefore the test substance is considered not ready biodegradable.
- Executive summary:
The purpose of this study was to determine the ready biodegradability in a manometric respirometric test over 28 days. The test item was exposed to activated sludge for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. Test method was according to OECD Guidline 301 F (Monometric Respirometry Test). Test substance (127.3 mg/L)) was added to culture medium (30 mg SS/L). As a reference item Sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control. Percentage degradation was calculated by dividing the amount of oxygen consumed per mg of test substance by ThOD. Biodegradation of test substance was 2.82% after 28 d treatment. Therefore the test substance is considered not ready biodegradable. The reference item Sodium benzoate was sufficiently degraded to a mean of 77.09 % after 14 days, and to a mean of 93.07 % after 28 days of incubation, based on ThOD.
In the toxicity control containing both, the test item and the reference item, a mean of 61.72 % biodegradation was noted.
The percentage biodegradation of the reference and toxicity item confirms the suitability of the used activated sludge inoculum.
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