Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 441-420-8 | CAS number: 113889-23-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of Cyclobutanate towards Daphnia magna was investigated according to OECD TG 202 under GLP. Daphnids were exposed to mean measured concentrations of 0.12, 0.26, 0.43, 0.68, 1.1, 2.1, 3.8, 6.6 and 12.4 mg/l for 48 hours under static conditions. The test material solutions were prepared by stirring an excess (100 mg/1) of test material in reconstituted water and then removing any undissolved test material by filtration. This "saturated" solution was then further diluted, as necessary, to provide the remaining test groups. Chemical analysis of the test solutions throughout the test showed measured test concentrations were between 81% and 123% of the nominal test concentrations at 0 and 48 hours. Although the test material was suspected to be volatile, no significant loss of test material was shown for each of the concentrations over the test period. It was considered that the use of completely sealed vessels with minimal headspace helped to prevent losses of the test material.
The results are based on the mean measured test concentrations as opposed to time-weighted mean measured test concentrations. The 48-Hour EC50 for the test material to Daphnia magna based on the mean measured test concentrations was 4.7 mg/l with 95% confidence limits of 4.1- 5.3 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 4.7 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
