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Long-term toxicity to aquatic invertebrates

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Description of key information

For Daphnia magna, a 21-day LOEC and NOEC of 3.2 and 1.6 mg/L WAF, respectively, based on immobilization was determined.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
1.6 mg/L

Additional information

A reliable long-term toxicity study with invertebrates is available for reaction mass of Amines, coco alkyl and ß-Alanine, N-(2-carboxyethyl)-, N-coco alkyl derivs. and ß-Alanine, N-coco alkyl derivs. and included in the dossier. 


Parr (2013) conducted a reliable (Klimisch 1) GLP compliant study following OECD 211 methods. Based on the results of a preliminary range-finding test, less than 24 hr old Daphnia magna were exposed (10 replicates of a single daphnid per group) to Water Accommodated Fractions (WAFs) of the test substance without solvent over a range of nominal loading rates of 0.20, 0.40, 0.80, 1.6 and 3.2 mg/L for a period of 21 days under semi-static test conditions.  The numbers of live and dead adult Daphnia and young daphnids (live and dead) were determined daily.  The Daphnia were fed daily with a mixture of algal suspension and Tetramin®flake food suspension. Study results indicate a 21-day LOEC and NOEC of 3.2 and 1.6 mg/L WAF, respectively, based on immobilization. Determination of NOEC based on reproduction was not possible due to test organism performance. Consequently, this study will be taken for the long-term toxicity of the test substance to invertebrates endpoint.