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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Description of key information

 The 96-h LC50 was 2.244 mg/L, based on mortality for a test with the Zebrafish, Danio rerio.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
2.244 mg/L

Additional information

A reliable short-term toxicity study with fish is available for reaction mass of Amines, coco alkyl and ß-Alanine, N-(2-carboxyethyl)-, N-coco alkyl derivs. and ß-Alanine, N-coco alkyl derivs. and included in this dossier.


Gancet & Haurat (2005) conducted a reliable (Klimisch 2) GLP compliant study according to OECD 203 methods. Juvenile Zebrafish,Danio reriowere exposed to nominal concentration of 0, 1.35, 1.75, 2.28, 2.96, 3.85 and 5 mg/L of the test substance in solvent for 96 hrs under static conditions.Measured concentrations were 96 to 121 % of nominal concentrations. The 96-h LC50 was 3.4 mg/L, based on mortality.Since the test substance was in 34% solvent, the actual effect concentration (96hr LC50), based on active ingredient was calculated to be 2.244 mg/L. Consequently this value will be taken as the short-term toxicity in fish endpoint for the test substance.