α,4-dichlorotoluene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16.10.1981 - 20.11.1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Materials and methods

Principles of method if other than guideline:

The fish were placed to the aquaria 96 hours before the test. The test substance was dissolved in water and was added to the test water. Duration of the test was 96 h. 10 fishes were exposed to 0; 1; 1.25; 1.6; 2; 2.24; 2.5; 4; 6, 3; 10 and 100 mg/L of 4-chlorobenzyl chloride. Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 2, 24, 72, and 96 hours to be within the acceptable limits.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

α,4-dichlorotoluene

EC Number:

203-242-7

EC Name:

α,4-dichlorotoluene

Cas Number:

104-83-6

Molecular formula:

C7H6Cl2

IUPAC Name:

1-chloro-4-(chloromethyl)benzene

Details on test material:

Assay:
99.5 % p-Chlorobenzyl chloride
0 -0.5 % p-Chlorobenzal chloride
0 -0.5 % p-Chlorobenzotrichloride
Appearance: Colourless liquid

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

Leuciscus idus f. melanotus
Source: Paul Eggers, Hohenwestedt
Weight: 1.4 - 2.6 g
Length: 5,4 - 6,7 cm

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

48 h and 96 h

Test conditions

Hardness:

192 mg/L NaHCO3
120 mg/L CaSO4 ·H2O
120 mg/L MgSO4

Test temperature:

19 -21 °C

pH:

pH 8.1 - 8.4

Dissolved oxygen:

> 8 mg/L

Salinity:

8 mg KCl

Nominal and measured concentrations:

0 mg/L, 1 mg/L, 1.25 mg/L, 1.6 mg/L, 2 mg/L, 2.24 mg/L, 2.5 mg/L, 4 mg/L, 6.3 mg/L, 10 mg/L, 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

2.24 - 2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2.24 - 2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Applicant's summary and conclusion

Conclusions:

From this study, LC50 for p-chlorobenzyl chloride was determined to be 2.24 - 2.5 mg/L.

Executive summary:

Testing laboratory was Deptm. Pharma Forschung, Toxikologie at Hoechst AG, Frankfurt/Main. A static test with nominal dissolved test substance concentrations was conducted. Duration of the test was 96 h. 10 test organisms were exposed to 0; 1; 1.25; 1.6; 2; 2.24; 2.5; 4; 6.3; 10 and 100 mg/L of 4-chlorobenzyl chloride. Water quality parameters (pH, temperature, oxygen content) were determined at time points 0, 2, 24, 72, and 96 hours to be within the acceptable limits. No lethality occurred in the control group.

At a concentration up to 1.6 mg/L no behavoural abnormalities were observed, no lethality occured till the end of the test.

The highest examined concentration without lethality is 2.24 mg/L after 48 h and 2 mg/L after 96 h.

The lowest examined concentration with 100 % lethality is 2.5 mg/L (after 48 h and 96 h).

As a result, the LC50 value is 2.24 -2.5 mg/L.