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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
fish, juvenile growth test
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well performed study. Effect concentrations based on measured selenium concentrations.

Data source

Reference
Reference Type:
publication
Title:
Toxicity of sodium selenite to rainbow trout fry
Author:
Hunn JB, Hamilton SJ, Buckler DR
Year:
1987
Bibliographic source:
Water Research 21, 233-238

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Salmo gairdneri (rainbow trout) fry were exposed to waterborne selenium as sodium selenite for a period of 90 days under flow-through conditions using a proportional diluter. Five concentrations and a control were tested. Test concentrations were analytically verified.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium selenite
EC Number:
233-267-9
EC Name:
Sodium selenite
Cas Number:
10102-18-8
Molecular formula:
H2O3Se.2Na
IUPAC Name:
disodium selenite
Details on test material:
- Name: sodium selenite
- Analytical purity: 98%
- Source: Sigma Chemical Company
- No further information stated

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Water samples were taken from all treatments, frequency not stated.

Test solutions

Vehicle:
no
Details on test solutions:
see test conditions

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: rainbow trout
- Source: no data
- Length at study initiation: 21.7 mm
- Weight at study initiation: 75 mg
- Fish were fed a commercial diet supplemented with live nauplii of brine shrimp (ad libitum) for the first 30 days of the study.

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
90 d

Test conditions

Hardness:
272 mg CaCO3/L
Test temperature:
12 +/- 1 °C
pH:
7.4
Dissolved oxygen:
237 mg CaCO3/L
Nominal and measured concentrations:
Measured concentrations: control, 7.8, 12.4, 21.0, 47.2 and 99.5 ug/L
Details on test conditions:
TEST SYSTEM
- Type of flow-through: proportional diluter
- No. of organisms per vessel: 20 fry
- No. of vessels per concentration (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: well water
- Dilution water parameters: Well water was characterized by pH 7.4, alkalinity 237 mg/L as CaCO3 , hardness 272 mg/L as CaCO3 and selenium < 0.4 ug/L.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, length and body weight

Results and discussion

Effect concentrationsopen allclose all
Duration:
90 d
Dose descriptor:
NOEC
Effect conc.:
21 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
element
Remarks:
Se
Basis for effect:
mortality
Duration:
90 d
Dose descriptor:
LOEC
Effect conc.:
47.2 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Se
Basis for effect:
mortality
Duration:
90 d
Dose descriptor:
NOEC
Effect conc.:
47.2 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
element
Remarks:
Se
Basis for effect:
length
Remarks:
and body weight
Duration:
90 d
Dose descriptor:
LOEC
Effect conc.:
99.5 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element
Remarks:
Se
Basis for effect:
length
Remarks:
and body weight
Reported statistics and error estimates:
Split plot in time analysis of variance + Least Significant Difference test.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Reliable (Klimisch 2) study in which Salmo gairdneri (rainbow trout) fry were exposed to selenium as sodium selenite for a period of 90 days under flow-through conditions using a proportional diluter. Five concentrations and a control were tested. Test concentrations were analytically verified. The most sensitive endpoint investigated was mortality with a 90-day NOEC and LOEC of 21 and 47.2 µg Se/L, respectively.