Ethyltriphenylfosfonium bromide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

as mentioned below

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of microorganism for the test chemical :
1) To evaluate Oxygen consumption of bacterial culture under the influence of test substance
2) Effect of test material was evaluated on Photobacterium sp. by luminescent bacteria test

GLP compliance:

not specified

Details on test solutions:

1) The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring

Test organisms (species):

other: 1) Pseudomonas putida 2)Photobacterium sp.

Details on inoculum:

1) - Strain : DSM 50026

Test type:

static

Water media type:

other: 1) algar slant

Total exposure duration:

30 min

Test temperature:

1) 25 °C.

Nominal and measured concentrations:

1) 156.25, 312.5, 625, 1250, 2500, 10000 mg/l.

Details on test conditions:

1) - Test vessle: test tube
-MEDIUM: 25 g/l nutrient broth I, 18 g/l Agar poured into slant-agar tubes.

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

100 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: Oxygen consumption

Duration:

15 min

Dose descriptor:

EC50

Effect conc.:

202 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

other: Bio Luminescence

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical is not likely to be toxic to microorganism atleast in the dose range of 202- 10000 mg/l

Executive summary:

Data available for the test chemicals has been reviewed to determine the toxicity of microorganism .The studies are as mentioned below:

1) The effect of test material on Pseudomonas putida was observed for its Oxygen consumption. Pseudomonas putida, strain DSM 50026 were cultured on 25 g/l nutrient broth , 18 g/l Agar poured into slant-agar tubes. The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring. The nominal test concentration used were 156.25, 312.5, 625, 1250, 2500, 10000 mg/l. The effect concentartion EC50 was after 30 min of eposure to test organism Pseudomonas putida was observed to be >10000 mg/l. Based on the above effect concentration it can be considered that test substance is not toxic for the microorganisms.

2) Effect of test material on bio luminescence property of Photobacterium sp. was evaluated after 15 min of exposure of test material. The EC50 value of test material on Photobacterium sp. after 15 mins was observed to be 202 mg/l. Based on the effect concentration it can be concluded that test material has no adverse effect on microorganism.

Description of key information

Toxicity to microorganism:

Data available for the test chemicals has been reviewed to determine the toxicity of microorganism .The studies are as mentioned below:

1) The effect of test material on Pseudomonas putida was observed for its Oxygen consumption. Pseudomonas putida, strain DSM 50026 were cultured on 25 g/l nutrient broth , 18 g/l Agar poured into slant-agar tubes. The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring. The nominal test concentration used were 156.25, 312.5, 625, 1250, 2500, 10000 mg/l. The effect concentartion EC50 was after 30 min of eposure to test organism Pseudomonas putida was observed to be >10000 mg/l. Based on the above effect concentration it can be considered that test substance is not toxic for the microorganisms.

2) Effect of test material on bio luminescence property of Photobacterium sp. was evaluated after 15 min of exposure of test material. The EC50 value of test material on Photobacterium sp. after 15 mins was observed to be 202 mg/l. Based on the effect concentration it can be concluded that test material has no adverse effect on microorganism.

Key value for chemical safety assessment

EC50 for microorganisms:

202 mg/L

Additional information

Toxicity to microorganism:

Data available for the test chemicals has been reviewed to determine the toxicity of microorganism .The studies are as mentioned below:

1) The effect of test material on Pseudomonas putida was observed for its Oxygen consumption. Pseudomonas putida, strain DSM 50026 were cultured on 25 g/l nutrient broth , 18 g/l Agar poured into slant-agar tubes. The stock solution was prepared with Tween 80 as dispersant and was stirred for 17 hours, resulting in a white suspension. The further dilutions were prepared from the stock solution while stirring. The nominal test concentration used were 156.25, 312.5, 625, 1250, 2500, 10000 mg/l. The effect concentartion EC50 was after 30 min of eposure to test organism Pseudomonas putida was observed to be >10000 mg/l. Based on the above effect concentration it can be considered that test substance is not toxic for the microorganisms.

2) Effect of test material on bio luminescence property of Photobacterium sp. was evaluated after 15 min of exposure of test material. The EC50 value of test material on Photobacterium sp. after 15 mins was observed to be 202 mg/l. Based on the effect concentration it can be concluded that test material has no adverse effect on microorganism.