Ethyltriphenylfosfonium bromide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of aquatic invertebrate for the test chemical :
1 and 2) Toxicity of test material on aquatic invertebrate Pomacea canaliculata was evaluated
3) To evaluate short term toxicity of aquatic invertebrate on test material

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: 1 and 2) Pomacea canaliculata , 3) Daphnia magna

Details on test organisms:

1 and 2) -common name: Golden apple snails

- Method of breeding: Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5,
26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.
The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Nominal and measured concentrations:

1 and 2) 0.5 to 250 mg/l

Details on test conditions:

1 and 2) - Test vessel: 500 mL glass bottles
-fill volume: 450 ml
- No. of organisms per vessel: 30
- No. of vessels per concentration (replicates): 6

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

16.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: linear regression (r2)= 2.441x + 8.742(0.942)

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

67.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: linear regression (r2)= 0.617 x + 8.382(0.932)

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

52.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical is likely to be toxic to aquatic invertebrate atleast in the concentration range of 16 mg/l to 67 mg/l

Executive summary:

Data available for the test chemical chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:

1) Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

 The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number  of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen.The lethal effect concentration ( LC50) was determined to be 16.9 mg/l(linear regression (r²)= 2.441x + 8.742(0.942)). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

 

2)Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity. The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

 The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number  of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen. The lethal effect concentration ( LC50) was determined to be 67.4 mg/l linear regression (r²)= 0.617 x + 8.382(0.932). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

3) Short term toxicity test for aquatic invertebrtae was performed for the test material for 24 h . The EC50 value was observed to be 52.6 mg/l , The EC0 was noted at 31.2 mg/l and the EC100 was observed in the concentration 125 mg/l.Based on the above effect concentartions it can be considered that test material was toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

Based on the above effect concentartions it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

 

Description of key information

Short term toxicity to aquatic invertebrates:

Data available for the test chemical chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:

1) Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

 The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number  of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen.The lethal effect concentration ( LC50) was determined to be 16.9 mg/l(linear regression (r²)= 2.441x + 8.742(0.942)). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

 

2)Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity. The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

 The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number  of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen. The lethal effect concentration ( LC50) was determined to be 67.4 mg/l linear regression (r²)= 0.617 x + 8.382(0.932). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

3) Short term toxicity test for aquatic invertebrtae was performed for the test material for 24 h . The EC50 value was observed to be 52.6 mg/l , The EC0 was noted at 31.2 mg/l and the EC100 was observed in the concentration 125 mg/l.Based on the above effect concentartions it can be considered that test material was toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

Based on the above effect concentartions it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

16.9 mg/L

Additional information

Short term toxicity to aquatic invertebrates:

Data available for the test chemical chemicals has been reviewed to determine the short term toxicity of aquatic invertebrate .The studies are as mentioned below:

1) Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity.The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

 The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number  of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen.The lethal effect concentration ( LC50) was determined to be 16.9 mg/l(linear regression (r²)= 2.441x + 8.742(0.942)). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

 

2)Short term toxicity to aquatic invertebrate Pomacea canaliculata (golden apple snail) was evaluated for the test material. Aquaria with dimensions of 50 cm x 25 cm x 35 cm were used for maintaining and breeding snails in the laboratory. The top of the aquarium was covered with nylon screen. Lamps were positioned 15 cm above the aquaria with a cycle of 16 hrs “on”, and 8 hrs “off’. The aquarium floor was covered with a layer of small pebbles, and was filled with water to a depth of 25 cm. Tap water reduced the thickness of the snail shell and the number of eggs, dechlorinated tap water caused the snails to secrete mucus. As a result, fresh aerated well water (pH 7.5, 26 °C) was provided in a continuous flow. Wild adult snails, with a diameter greater than 5 cm, were collected from the field. After copulation, fresh eggs were incubated and the young snails were hatched in 10-17 days. The young snails were fed sweet potato leaves in the aquarium for 65-70 days until maturity. The breeding was repeated and the third generation snails of 35-40 days old were used for toxicity testing.

 The bioassay was conducted in 500 mL glass bottles, each was tilled with thirty snails and 450 mL of water. The concentration of test compounds ranged from 0.5 to 250 mg/L (6 different concentrations, three replications). After 72 hrs exposure, the number  of dead snails were recorded. The snail was considered to be dead if there was no response to touch, the operculum shut tight, but with no resistance when the operculum was lifted, and the foot was out and swollen. The lethal effect concentration ( LC50) was determined to be 67.4 mg/l linear regression (r²)= 0.617 x + 8.382(0.932). Based on the above effect concentration it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

3) Short term toxicity test for aquatic invertebrtae was performed for the test material for 24 h . The EC50 value was observed to be 52.6 mg/l , The EC0 was noted at 31.2 mg/l and the EC100 was observed in the concentration 125 mg/l.Based on the above effect concentartions it can be considered that test material was toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.

Based on the above effect concentartions it can be considered that the test substance is toxic to aquatic invertebrate and can be classified as aquatic chronic 3 as per CLP criteria.