Registration Dossier
Registration Dossier
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EC number: 246-836-1 | CAS number: 25321-14-6
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dinitrotoluene
- EC Number:
- 246-836-1
- EC Name:
- Dinitrotoluene
- Cas Number:
- 25321-14-6
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,4-dinitrobenzene; 2-methyl-1,3-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): DNT 80/20 (= DNT isomers mixture)
- The CAS-No. of 2,4-DNT is incorrectly reported to be the CAS-No. of DNT 80/20 (DNT isomers mixture, CAS-No. 25321-14-6).
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test substrate
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- Eisenia fetida andrei
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20 +/- 1 °C
- pH:
- 6.0 +/- 0.5
- Moisture:
- 70 - 90% (rel. humidity)
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1.5 L
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture
- % sand: 70
- % peat: 10 (pH 6+/-0.5)
- % kaolin: 20
OTHER TEST CONDITIONS
- Photoperiod: constant
- Light intensity: 400 - 800 lux
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 316 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 668 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: CI: 622-731 mg/kg dw
- Duration:
- 14 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 562 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
