Dinitrotoluene

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, study was performed similar to German Industrial Standard (DIN), peer reviewed data (OECD SIDS (2004)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda)

Author:

Bringmann, G. and Kühn, R.,

Year:

1977

Bibliographic source:

Z. f. Wasser- und Abwasser-Forschung 10, 87-98

Reference 2

Reference Type:

secondary source

Title:

Dinitrotoluene (isomers mixture), CAS No.: 25321-14-6

Author:

OECD SIDS

Year:

2004

Bibliographic source:

UNEP publications

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

Principles of method if other than guideline:

inhibition of cell multiplication according to Bringmann and Kuehn

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,4-dinitrotoluene

EC Number:

204-450-0

EC Name:

2,4-dinitrotoluene

Cas Number:

121-14-2

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,4-dinitrobenzene

Constituent 2

Reference substance name:

2,6-dinitrotoluene

EC Number:

210-106-0

EC Name:

2,6-dinitrotoluene

Cas Number:

606-20-2

Molecular formula:

C7H6N2O4

IUPAC Name:

2-methyl-1,3-dinitrobenzene

Constituent 3

Reference substance name:

2,3-dinitrotoluene

EC Number:

210-013-5

EC Name:

2,3-dinitrotoluene

Cas Number:

602-01-7

Molecular formula:

C7H6N2O4

IUPAC Name:

1-methyl-2,3-dinitrobenzene

Details on test material:

- Test substance:
2,4-Dinitrotoluene (2,4-DNT), CAS No. 121-14-2
2,6-Dinitrotoluene (2,6-DNT), CAS No. 606-20-2
2,3-Dinitrotoluene (2,3-DNT), CAS No. 602-01-7
- Analytical purity: not stated

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

16 h

Test conditions

Test temperature:

25 °C

pH:

7.0

Details on test conditions:

Type: aquatic

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

16 h

Dose descriptor:

other: toxicity threshold (TT)

Effect conc.:

57 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,4-DNT

Basis for effect:

other: Cell multiplication inhibition

Remarks on result:

other: TT (toxicity threshold) comparable to EC3

Effect concentrations 2

Duration:

16 h

Dose descriptor:

other: toxicity threshold (TT)

Effect conc.:

26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,6-DNT

Basis for effect:

other: Cell multiplication inhibition

Remarks on result:

other: TT (toxicity threshold) comparable to EC3

Effect concentrations 3

Duration:

16 h

Dose descriptor:

other: toxicity threshold (TT)

Effect conc.:

9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

2,3-DNT

Basis for effect:

other: Cell multiplication inhibition

Remarks on result:

other: TT (toxicity threshold) comparable to EC3