Reaction mass of 3-(octanoyloxy)propyl decanoate and propane-1,3-diyl didecanoate and propane-1,3-diyl dioctanoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11. - 13.04.1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

EG-Richtlinie 92/69/EWG

Principles of method if other than guideline:

Highest and only measured concentration: 2.7 mg/L

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

No details available.

Vehicle:

no

Details on test solutions:

The test item was prepared with deionised water (1g/L) and shaked for 18h. Thereafter the solution was filtrated and the DOC value determined.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus, clone 5
- Age at study initiation: 24h
- Source: own breeding
- Age of parental stock: 2-3 days
- Feeding during test : no

ACCLIMATION
- Acclimation period: no, same as test conditions

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19 - 21 °C

pH:

7.8 - 8.1

Dissolved oxygen:

8.2 - 8.5 mg/L

Nominal and measured concentrations:

Nominal concentration: 2.7 mg/L
Measured concentration: 2.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: erlenmeyer flask
- Material: glass
- Volume of solution: 10 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2mL/animal

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to EG Richt1inie 92/69/EWG
CaC12 x 2 H2O: 294 mg/L
MgSO4 x 7 H2O: 123 mg/L
NaHCO3: 63 mg/L
KCl: 5.5 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: dark

EFFECT PARAMETERS MEASURED: Immobility (after 24h and 48h)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no

Results with reference substance (positive control):

- Results with reference substance valid? yes
Two concentrations were measured: At 1 mg/L 35% of the Daphnids were immobilized, at 2 mg/L 100% of the Daphnids were immobilized.

Validity criteria fulfilled:

yes

Conclusions:

In the Daphnia immbilisation test a EC50 value > 2.7 mg/L was determined.

Executive summary:

The toxicity of the test item to Daphnia magna was determined according to the principles of OECD 202. The aim of the study was the determination of NOEC, LOEC, EC10- , EC20- and EC50-values of immobilsation over a period of 48 hours. The study was conducted under static conditions. A stock solution of 1 g/L was prepared with deionised water. One concentration was tested: 2.7 mg/L. Five replicates were tested for the test item concentration and for the control. The environmental conditions were within the acceptable limits. The concentrations of the test item in the test concentration and the control were analytically verified by TOC Infrared analyser at the start and the end of exposure. The measured concentrations were in the range of 20 % of the nominal value. Therefore, all effect values given are based on the nominal test item concentrations. No immobilistion was observed. The following results were determined: EC50 >2.7 mg/L, NOEC = 2.7 mg/L, LOEC > 2.7 mg/L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

12. - 15.05.1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

EG-Richtlinie 92/69/EWG

Principles of method if other than guideline:

Highest and only measured concentration: 2.0 mg/L

GLP compliance:

yes (incl. QA statement)

Remarks:

853947-59-8

Analytical monitoring:

no

Details on sampling:

The analytical determination of the stability of the test item was tested separately. Concentrations of 0.5, 1.2 and 2.4 mg/L were analyzed after 24, 48 and 72 h. The recovery rate was >80%, therefore nominal values were used for the test. Determination of Carbon with a TOC-500 Infrared analyser. No additional details available. During the test no concentrations were measured.

Vehicle:

no

Details on test solutions:

The test item was prepared with deionised water (1g/L) and shaked for 18h. Thereafter the solution was filtrated and the DOC value determined.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Straus, clone 5
- Age at study initiation: 24h
- Source: own breeding
- Age of parental stock: 2-3 days
- Feeding during test : no

ACCLIMATION
- Acclimation period: no, same as test conditions

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19 - 21 °C

pH:

7.9 - 8.0

Dissolved oxygen:

7.7 - 8.1 mg/L

Nominal and measured concentrations:

Nominal concentration: 2.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: erlenmeyer flask
- Material: glass
- Volume of solution: 10 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 2mL/animal

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to EG Richt1inie 92/69/EWG
CaC12 x 2 H2O: 294 mg/L
MgSO4 x 7 H2O: 123 mg/L
NaHCO3: 63 mg/L
KCl: 5.5 mg/L

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: dark

EFFECT PARAMETERS MEASURED: Immobility (after 24h and 48h)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: no
- Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: no

Results with reference substance (positive control):

- Results with reference substance valid? yes
Two concentrations were measured: At 1 mg/L 40% of the Daphnids were immobilized, at 2 mg/L 100% of the Daphnids were immobilized.

Validity criteria fulfilled:

yes

Conclusions:

In the Daphnia immbilisation test a EC50 value > 2.0 mg/L was determined.

Executive summary:

The toxicity of the test item to Daphnia magna was determined according to the principles of OECD 202. The aim of the study was the determination of NOEC, LOEC, EC10- , EC20- and EC50-values of immobilsation over a period of 48 hours. The study was conducted under static conditions. A stock solution of 1 g/L was prepared with deionised water. One concentration was tested: 2.0 mg/L. Five replicates were tested for the test item concentration and for the control. The environmental conditions were within the acceptable limits. The analytical determination of the stability of the test item was tested separately. Concentrations of 0.5, 1.2 and 2.4 mg/L were analyzed after 24, 48 and 72 h. The recovery rate was >80%, therefor nominal values were used for the test. During the test no concentrations were measured. No immobilistion was observed. The following results were determined: EC50 >2.0 mg/L, NOEC = 2.0 mg/L, LOEC > 2.0 mg/L.

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to chapter 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 2.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to chapter 13.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

> 2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Description of key information

No data on the test item is available. With the read-across substances the following results were obtained:

CAS 68583-51-7: EC50 >2.7 mg/L, NOEC = 2.7 mg/L

CAS 853947-59-8:EC50 >2.0 mg/L, NOEC = 2.0 mg/L

Additional information

No data on the test item is available. With the read-across substances the following results were obtained:

The toxicity of the read-across substance CAS 68583-51-7 to Daphnia magna was determined according to the principles of OECD 202. The aim of the study was the determination of NOEC, LOEC, EC10- , EC20- and EC50-values of immobilsation over a period of 48 hours. The study was conducted under static conditions. A stock solution of 1 g/L was prepared with deionised water. One concentration was tested: 2.7 mg/L. Five replicates were tested for the test item concentration and for the control. The environmental conditions were within the acceptable limits. The concentrations of the test item in the test concentration and the control were analytically verified by TOC Infrared analyser at the start and the end of exposure. The measured concentrations were in the range of 20 % of the nominal value. Therefore, all effect values given are based on the nominal test item concentrations. No immobilistion was observed. The following results were determined: EC50 >2.7 mg/L, NOEC = 2.7 mg/L, LOEC > 2.7 mg/L.

The toxicity of the read-across substance 853947-59-8 to Daphnia magna was determined according to the principles of OECD 202. The aim of the study was the determination of NOEC, LOEC, EC10- , EC20- and EC50-values of immobilsation over a period of 48 hours. The study was conducted under static conditions. A stock solution of 1 g/L was prepared with deionised water. One concentration was tested: 2.0 mg/L. Five replicates were tested for the test item concentration and for the control. The environmental conditions were within the acceptable limits. The analytical determination of the stability of the test item was tested separately. Concentrations of 0.5, 1.2 and 2.4 mg/L were analyzed after 24, 48 and 72 h. The recovery rate was >80%, therefor nominal values were used for the test. During the test no concentrations were measured. No immobilistion was observed. The following results were determined: EC50 >2.0 mg/L, NOEC = 2.0 mg/L, LOEC > 2.0 mg/L.