Ammonium propionate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium propionate

EC Number:

241-503-7

EC Name:

Ammonium propionate

Cas Number:

17496-08-1

Molecular formula:

C3H6O2.H3N

IUPAC Name:

ammonium propionate

Test material form:

liquid

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0 - 20.1 ºC

pH:

Start: 6.60 - 7.48
End: 7.49 - 7.50

Dissolved oxygen:

test start: 6.84 – 7.52 mg O2/L
Test end: 6.63 – 6.43 mg O2/L

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

The test was performed in glass beakers filled with 40 mL of test medium. The test vessels were covered with glass plates to reduce the loss of water and to avoid dust entry into test solutions.
The test vessels were labelled with the internal study number and all necessary additional information to ensure unmistakable identification.
For the tested concentration and for the negative control, group of 20 daphnids were used divided into four replicates of five daphnids each. The daphnids were randomly distributed to the test vessels at test initiation. The daphnids loading rate was about 8 mL per one daphnid (5 daphnids for 40 mL, according to OECD guideline 202, 2004).


Temperature:
The incubation temperature was continuously monitored during the course of the study by a PT100 probe installed in the environmental test chamber. It was in the range 20.0 – 20.1°C with a mean value of 20.0 °C and a standard deviation of 0.02 °C. The temperature range provided by OECD guideline 202, 2004 is 20 ± 2°C. The temperature values during the test were within the provided range and did not vary more than 1°C during the test period.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance is not classified as toxic for the aquatic invertebrates