Ammonium propionate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Title:

Unnamed

Year:

2017

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium propionate

EC Number:

241-503-7

EC Name:

Ammonium propionate

Cas Number:

17496-08-1

Molecular formula:

C3H6O2.H3N

IUPAC Name:

ammonium propionate

Test material form:

liquid

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, adapted

Details on inoculum:

The study was performed using aerobic activated sludge from BRIANZACQUE civil wastewater treatment plant. The sludge was collected on November 17, 2017. A homogenized aliquot of the wet sludge was centrifuged, the supernatant was removed while the solid phase was weighed, dried and the ratio of wet to dry weight was calculated to be equal to 6.36%.
Based on this ratio, calculated amounts of wet sludge was centrifuged, the supernatant was removed and the solid phase was suspended in the test medium to get a concentration equivalent to 3 g dry material per litre.
The prepared sludge inoculum was pre-conditioned to the experimental conditions by aerating it in the dark. The concentrated suspension was used as inoculum to give a final concentration of 15 mg dry material per litre into test flasks

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 68 mg/L

Based on:

COD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

ca. 90.7

Sampling time:

28 d

Details on results:

The biodegradation was followed by measuring the oxygen uptake (mg/L) of the test item under defined conditions by the means of a manometric method and calculating from these values the BOD (Biochemical Oxygen Demand)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test item, Ammonium propionate (CAS: 17496-08-1; EC: 241-503-7) in aqueous solution (50-55 %), was found to be ready and completely biodegradable under the conditions applied in a manometric respirometry test, having showed a maximum biodegradation of 91.9% at the end of the 10-day window and a maximum biodegradation of 105.9% at the end of the test (mean percentage values between two replicates), but after correction with nitrification contribution the corrected value of biodegradation at the end of the test period was 90.7%

No significant degradation was observed in the abiotic control.

The test item showed no inhibitory effects on the microorganism activity at the tested concentration, as demonstrated by the biodegradation percentage in the toxicity control exceeding the 25% pass level after two days of exposure (37.1%).