Phosphoric acid, butyl ester, sodium salt

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

REPORTING FORMAT FOR THE ANALOGUE APPROACH

Please refer to the attached read-across justification in section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992-10-12 to 1992-10-16

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

- Molecular weight: 210.2 g/mol

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Application of test substance: direct weighing.
Undissolved particles of test substance sedimented on the ground of the vessel, but were dissolved after 15 minutes.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Origin: West-Aquarium (Bad Lauterberg, Germany)
- Body length: 2.5 - 3.5 cm
- Mortality during cultivation: < 2 %
- Culture water (medium): synthetic fresh water in arccordance with ISO

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

10.9 °dH

Test temperature:

20.9 - 21.4 °C

pH:

7.2 - 8.2

Dissolved oxygen:

7.8 - 9.1 (90.5 - 104.2 %)

Nominal and measured concentrations:

Nominal conc: 100 mg/L (direct weighing), no measured concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Material, size, fill volume: glass, 300*135*200 mm, 5 L test medium
- Aeration: yes, air circulation
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light : 8 h darkness

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was observed. After 2 hours, the test item-treated animals appeared somewhat lethargic.

Results with reference substance (positive control):

no positive control was tested.

Sublethal observations / clinical signs:

Validity of the test

No mortality in the control occured during the test. The dissolved oxygen concentration remained above 90 % throughout the exposure period.

Table 1: abiotic parameters, test item

	0 h	24 h	48 h	72 h	96 h
Temperature	21.4	20.9	20.7	21.2	21.2
Oxygen [mg/L]	7.8	8.6	9.1	8.3	8.2
Oxygen saturation [%]	90.5	98.9	104.2	96	94.8
pH	8.2	7.2	7.3	7.2	7.6

 

Table 2: abiotic parameters, control

	0 h	24 h	48 h	72 h	96 h
Temperature	21.3	20.8	20.7	21.2	21.2
Oxygen [mg/L]	7.8	8.8	9.2	8.5	8.4
Oxygen saturation [%]	90.3	101	105.4	98.3	97.1
pH	8.2	7.7	7.7	7.4	7.5

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of the test item on zebrafish was determined to be >100 mg/L after 96 hours exposure.

Executive summary:

The acute toxicity of the test item to fish was conducted in a static test according to OECD 201 and EU Method C.1 (Acute Toxicity for Fish). 10 zebrafish (Danio rerio) were exposed to a limit concentration of 100 mg/L. The test solution was prepared by direct weighing of the test item in 5 L test medium. A concurrent control ran parallel. After 96 hours of exposure a LC0 value of ≥ 100 mg/L was obtained. The LC50 was determined to be >100 mg/L. No significant effects of the test item on the fish were observed. All reported values are based on the nominal concentration.

Description of key information

The LC50 on zebrafish was determined to be >100 mg/L after 96 hours exposure.

Additional information

No study on fish with the target substance is available. A read-across to Dibutyl hydrogen phosphate (CAS 107-66-4) was performed to address the endpoint of short-term toxicity to fish.

The acute toxicity of the source item to fish was conducted in a static test according to OECD 201 and EU Method C.1 (Acute Toxicity for Fish). 10 zebrafish (Danio rerio) were exposed to a limit concentration of 100 mg/L. The test solution was prepared by direct weighing of the source item in 5 L test medium. A concurrent control ran parallel. After 96 hours of exposure a LC0 value of ≥ 100 mg/L was obtained. The LC50 was determined to be >100 mg/L. No significant effects of the test item on the fish were observed. All reported values are based on the nominal concentration.

In conclusion, the source substance showed no significant toxic effects on the tested animals. Therefore, it is expected that the target substance has no significant toxic effects on fish and the LC50 after 96 h exposure is >100 mg/L.