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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

There is no test performed with cyclohexylidenebis[tert-amyl] peroxide. Ready biodegradability of this substance is read-across from data obtained with cyclohexylidenebis[tert-butyl] peroxide: the peroxide was not biodegraded in the 28 -day modified Sturm test and should therefore not be classified as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

There is no test performed with cyclohexylidenebis[tert-amyl] peroxide. Ready biodegradability of this substance is read-across from data obtained with cyclohexylidenebis[tert-butyl] peroxide.

The ready biodegradability of cyclohexylidenebis[tert-butyl] peroxide was evaluated using a 28 -day modified Sturm test according to EU method C.4 -C and OECD guideline no. 301 B.

The flasks of the test item obtained a peroxide concentration of 10 mg/L of TOC and inoculum. The inoculum consisted of sewage sludge sampled from the aeration tank of a sewage treatment plant and was aerated for 7 days. Inoculum concentration was 12.0 mg/L (dry weight) in all test vessels. CO2 scrubbed air was bubbled through the flasks for the 28 day test period.

The biodegradation of cyclohexylidenebis[tert-butyl] peroxide totaled 5 % at the end of the test. Therefore, cyclohexylidenebis[tert-butyl] peroxide was considered as not readily biodegradable in the 28 -day modified Sturm test.