Docosyl methacrylate

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 428 (Skin Absorption: In Vitro Method)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-Propenoic acid, 2-methyl-, C12-16-alkyl esters

EC Number:

292-094-7

EC Name:

2-Propenoic acid, 2-methyl-, C12-16-alkyl esters

Cas Number:

90551-76-1

IUPAC Name:

2-Propenoic acid, 2-methyl-, C12-16-alkyl esters

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 780803361

Radiolabelling:

no

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

48 hrs

Doses:

100 µL/ cm2

Details on study design:

The absorption of Lauryl methacrylate was evaluated through rat epidermis and intact skin in an in vitro system. Values for human skin absorption
were extrapolated on the basis of a 14 times more efficient permeation through rat skin obtained in an epidermal PBPK model developed in this study.

Results and discussion

Signs and symptoms of toxicity:

not examined

Dermal irritation:

not examined

Absorption in different matrices:

Absorption of Lauryl methacrylate through rat epidermis:

The substance is readily absorbed through rat epidermis at a constant mean rate of 26.2 µg cm-2 / h.
This rate of absorption is virtually constant over the duration of the experiment.
The total amount of chemical that was absorbed during the time of exposure was 0.7 % of the applied dose.

Absorption of Lauryl methacrylate through whole rat skin:

Like the smaller esters investigated, Lauryl methacrylate was also metabolised to methacrylic acid as it was absorbed through whole rat skin. The peak rate of appearance of methacrylic acid was calculated to be 11.8 µg cm-2 / h, which occurred between 8 and 24 hours.
0.264 % of the Lauryl methacrylate was depleted from the donor reservoir over the 24 hr exposure period.

Percutaneous absorption

Percutaneous absorption 1

Key result

Time point:

24 h

Dose:

100 µL/cm2

Parameter:

percentage

Absorption:

0.7 %

Remarks on result:

other: Rat epidermis

Percutaneous absorption 2

Key result

Time point:

24 h

Dose:

100 µL/cm2

Parameter:

percentage

Absorption:

0.264 %

Remarks on result:

other: whole rat skin

Conversion factor human vs. animal skin:

With respect to human skin, 14 times more efficient permeation through rat skin obtained in an epidermal PBPK model developed in this study.