Carbamic acid, (diaminophosphinyl)-, 1-methylethyl ester

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006-08-16 - 2006-12-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

isopropyl (diaminophosphoryl) carbamate

Cas Number:

298692-41-8

Molecular formula:

C4H12N3O3P

IUPAC Name:

isopropyl (diaminophosphoryl) carbamate

Constituent 2

Reference substance name:

Carbamic acid, (diaminophosphinyl)-1-methylethylester

IUPAC Name:

Carbamic acid, (diaminophosphinyl)-1-methylethylester

Details on test material:

Purity: > 99%
batch no.: S-9

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

microbial count in the test flasks at test start: 8.3 e5 CFU/l

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

40 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

0

Sampling time:

28 d

Details on results:

There was no marked reduction in DOC in the test item solutions and the abiotic control. For the reference substance controls DOC concentrations were analysed that indicated continuously DOC decrease. When the values of the reference substance were in the range of the inoculum control the measurements were stopped.

BOD5 / COD results

Results with reference substance:

Within 6 days the reference compound was degraded almost completely. The pass level for ready biodegradability (70 %) was reached on the 3th day. The result is a criteria for normal test run.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

No marked DOC removal in the test item solutions was measured. The pass level for ready biodegradation (70 %) was not reached. For this reason the test item seems to be not readily biodegradable.
The result was not caused by inhibition of the inoculum by the test item. The measurements of the toxicity control showed that the test item had no inhibitory effect to bacteria.

Executive summary:

No marked DOC removal in the test item solutions was measured. The pass level for ready biodegradation (70 %) was not reached. For this reason the test item seems to be not readily biodegradable. The result was not caused by inhibition of the inoculum by the test item. The measurements of the toxicity control showed that the test item had no inhibitory effect to bacteria.