Benzenesulfonic acid, 2-[2-[5-(aminocarbonyl)-1-ethyl-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridinyl]diazenyl]-4-[[4-chloro-6-[[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-, sodium salt (1:2)

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

12 June 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study conducted to EU & OECD test guidance in compliance with GLP. This study is read across to the structural analogue Reaktiv-Gelb F-97494, which is considered to be structurally equivalent to the substance to be registered. Details of structure are detailed below under "illustrations"

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Test material information

Constituent 1

Details on test material:

See below

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: > 300 deg C
- Boiling point: Not measured
- Vapour pressure: < 10E-05 hPa
- Water solubility (under test conditions): 108 g/l
- Solubility in organic solvents: Not measured.
- log Pow: < -4.9
- ready biodegradation: 8% after 28 days
- Toxicity to microorganisms: EC50 > 10000 mg/l

Radiolabelling:

no

Study design

Test temperature:

No data

HPLC method

Details on study design: HPLC method:

The chromatographic conditions were the same for the determination of to, the establishment of the calibration graph and the measurement of the test substance.

HPLC equipment: HPLC pumpG1311A, autosampler G1313A, column oven G1316A and UV/VIS detector G1314A by Agilent
Column: Material: stainless steel, dimensions: 125 mm x 4.6 mm by Duratec (18904-02)
stationary phase: LiChrospher 100 CN 5//m
column temperature: 20°C
Mobile Phase: methanole / buffer pH 8.0
25 :75 (v/v)
isocratic elution
Flow rate: 1.0 ml/min
Injection volume: Dead time marker: 10μl
Test substance: 25μl
Calibration substances (without Direct Red): 10μl
Direct Red and test substance: 100μl
Detection wavelength: Dead time marker: 220nm
Calibration substances (without Direct Red): 220nm
Direct Red: 395nm
Test substance: 290nm
Injection amounts: Dead time marker: 2.72μg
Calibration substances: 0.71 – 7.42μg
Test substance: 3.91μg

The dead time marker was injected twice as solutions in acetonitrile/distilied water (50:50) (v/v). The calibration substances (without Direct Red) were dissolved in acetonitrile and diluted 0.1 to 1 in the mobile phase and injected as single standards twice. Direct Red and the test substance were dissolved in distilled water and diluted 0.1 to 1 in buffer pH 8 and injected as single solutions twice.

Batch equilibrium or other method

Analytical monitoring:

not required

Details on sampling:

Not applicable

Details on matrix:

Not applicable

Details on test conditions:

Not applicable

Computational methods:

Not applicable

Results and discussion

Adsorption coefficient

Type:

log Koc

Value:

-0.24

Remarks on result:

other: SD 0.12, RSD 50%

Results: HPLC method

Details on results (HPLC method):

Tables of results listed under any other information

Results: Batch equilibrium or other method

Adsorption and desorption constants:

Not applicable

Recovery of test material:

Not applicable

Concentration of test substance at end of adsorption equilibration period:

Not applicable

Concentration of test substance at end of desorption equilibration period:

Not applicable

Any other information on results incl. tables

Log K_ocof the Test Substance – 1. Measurement

Test substance	1. Inj. tRin min	k	Log k	Log Koc
Reacktiv Gelb F-97494	1.99	0.11	-0.96	-0.16

 

Log K_ocof the Test Substance – 2. Measurement

 

Test substance	2. Inj. tRin min	k	Log k	Log Koc
Reaktiv Gelb F-97494	1.94	0.08	-1.10	-0.32

 

Log K_ocof the Test Substance

 

	Log Koc
1. Measurement	-0.16
2. Measurement	-0.32
Mean	-0.24
Standard deviation	0.12
RSD%	50

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test substance has been determined according to the "HPLC- method" described in guideline 67/548/EEC, appendix C.19.

It was found to be:
log Koc: -0-24
Standard deviation: 0.12
RSD: 50%

Executive summary:

The objective of this study was the determination of the adsorption coefficient Koc of the test substance according to guideline 67/548/EEC, last change dated August 06, 2001, Annex Part C:Methods for determination of ecotoxicity. C.19. estimation of the adsorption coefficient on soil and on sewage sludge using HPLC and OECD test guidelines 121, estimation of the adsorption coefficient on soil and on sewage sludge. Study performed in compliance with the Principles of Good Laboratory Practice (GLP) and reported with a GLP certificate.

 

The adsorption coefficient of the test substance by analogue of read across has been determined.

 

It was found to be:

logKoc-0-24

Standard deviation: 0.12

RSD: 50%