Benzenesulfonic acid, 2-[2-[5-(aminocarbonyl)-1-ethyl-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridinyl]diazenyl]-4-[[4-chloro-6-[[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-, sodium salt (1:2)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

17 October 2001 to 14 November 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study conducted to EU & OECD test guidance in compliance with GLP. This study is read across to the structural analogue Reaktiv-Gelb F-97494, which is considered to be structurally equivalent to the substance to be registered. Details of structure are detailed below under "illustrations"

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

See below

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: > 300 deg C
- Boiling point: Not measured
- Vapour pressure: < 10E-05 hPa
- Water solubility (under test conditions): 108 g/l
- Solubility in organic solvents: Not measured.
- log Pow: < -4.9
- Adsorption characteristics:
- Toxicity to microorganisms: EC50 > 10000 mg/l

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Type: mixed population of aquatic micro organisms(activated sludge)
Origin: aeration tank of a waste water plant treating predominantly domestic sewage (Klaranlage Odenthal)
Date of collection: 2001-10-15
Pre-treatment: none
Concentration of inoculums: 30mg/l SS

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Pretreatment of the test substance: 25 mg test substance were weighed out on aluminium foil. This substance inclusive the aluminium foil was added to the test vessels to give a test concentration of 100 mg/l test substance.
Reference compound: Sodium benzoate with a purity of 99 % was used as reference compound.
Exposure conditions
Test volume: 250 ml
Test apparatus: Voitli Sapromat
Mixing: 1 magnetic stirrer per test vessel
Incubation time: 28 days
Incubation temperature: 20 ± 1°C

Reference substance

Reference substance:

other: Sodium benzonate

Results and discussion

Preliminary study:

Not applicable

Test performance:

The test substance and reference substance performed within the accepted parameters specified by the guideline.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

8

Sampling time:

28 d

Details on results:

All validity criteria of the test method were met:
The difference of extremes of replicate values of the removal of test chemical at the end of the test is less than 20 %.
The oxygen uptake of the inoculum blank < 60 mg/l.
The pH - 6.5 - 8.5 in the test vessels at the end of the test (if degradation is less than 60 %).
Oxygen uptake by nitrification has been determined. The oxygen consumed by nitrification was 6 mg/l. This oxygen consumption by nitrification has been substracted from the respective 28 days measurements of the test substance.

BOD5 / COD results

BOD5 / COD

Parameter:

BOD5

Value:

915 mg O2/g test mat.

Results with reference substance:

The reference compound has reached the level for ready biodegradabilily by 14 days. No toxicity of the test substance has been observed in the toxicity control.

Any other information on results incl. tables

Test substance
Time (days)		2	6	8	12	14	16	20	22	26	28
Corrected BOD	a1	1	3	3	6	6	5	6	9	11	6
Test substance (a-bm)	a2	2	4	5	7	7	8	8	10	12	7
[mg O2/l]	a3	3	5	6	7	8	8	9	11	13	8
% degradation	a1	1	3	3	7	7	5	7	10	12	7
	a2	2	4	5	8	8	9	9	11	13	8
	a3	3	5	7	8	9	9	10	12	14	9
% degradation, mean		2	4	5	8	8	8	9	11	13	8

Comments: pH of the test vessels at the end of the test:

a1: pH = 7.5

a2: pH = 7.5

a3: pH = 7.5

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Within 28 days, a degradation of 8 % was determined tor Reaktivgelb F97-494.

Reaktivgelb F97-494 has to be classified as "Not Readily Biodegradable".

Executive summary:

A study was performed to assess the ready biodegradabilily of Reaktivgelb F97-494.

 

The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". This test method is in all essential parts identical with OECD Guideline 301 F. Study performed in accordance with the Principles of Good Laboratory Practice (GLP).

 

A solution ofReaktivgelb F97-494 in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period the ready biodegradability is determined.

 

Reaktivgelb F97-494showed:

2 % degradation after 2 days.

4 % degradation after 6 days.

5 % degradation after 8 days.

8 % degradation after 12 days.

8 % degradation after 14 days.

8 % degradation after 16 days.

9 % degradation after 20 days.

11 % degradation after 22 days.

13 % degradation after 26 days.

8 % degradation after 28 days

 

Therefore the test substance, by analogy of read across has to be classified as "Not Readily Biodegradable".

 

The reference compound showed 90 % degradation alter 14 days.