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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

the test item Reaction mass of CXN1-55 was found readily biodegradable under the test conditions

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

In a valid, reliable and conclusive study, the test item Reaction mass of CXN1-55 was investigated for its ready biodegradability according to the OECD Guideline for Testing of Chemicals, No. 301 A (1992), the Commission Regulation (EC) No 440/2008 C.4-A and OPPTS 835.3110.

In the test flasks, containing the test item Reaction mass of CXN1-55 and activated sludge (inoculum), the mean concentrations of dissolved organic carbon (DOC) rapidly decreased to 79 % of the initial values by Exposure Day 5, reaching 99 % biodegradation within the test period of 21 days. The pass level for ready biodegradability (70 % removal of DOC in a 10-day window within a 28-day period) was reached within five days of exposure. The DOC content measured on Day 0 at test start (0h, with 29.6 mg/L) remained constant after 3 hours (Day 0, 3h, with 28.8 mg/L) indicating that no adsorption of the test item to the sludge occurred in this time.

In conclusion, the test item Reaction mass of CXN1-55 was found readily biodegradable under the test conditions.

The reference item sodium benzoate was readily biodegraded by an average of 98 % within two days of exposure, thus confirming suitability of the activated sludge

In the toxicity control, containing the test item, the reference item sodium benzoate and activated sludge (inoculum), the initial DOC decreased by 100% within 14 days of exposure. Thus, according to the test guidelines the test item was not inhibitory to activated sludge at the tested concentration of 90 mg/L because degradation was >35% within 14 days.