Cyclopropyl methyl ketone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Cyclopropyl methyl ketone

EC Number:

212-146-4

EC Name:

Cyclopropyl methyl ketone

Cas Number:

765-43-5

Molecular formula:

C5H8O

IUPAC Name:

1-cyclopropylethan-1-one

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)

- Origin: the water flea Daphnia magna Straus used is that of clone 5. Originally, the animals were received 1991 from Dr. N. Caspers (Bayer AG,
Leverkusen, Germany)

- Culture conditions: For breeding, the females are maintained in M4 medium in 1 L beakers. The daily amount of food, comprising Desmodesmus
subspicatus, is such that the amount added is consumed by the animals. A reddish dark-brown coloration of the animals indicates that they are
healthy. At two to three day intervals, the exuviae are syphoned off and the water is changed. In the course of this, the offspring is removed from the breeding vessels. At regular intervals of approximately 4 weeks, offspring is isolated from the vessels. They serve as a basis for further breeding.
The culture conditions ensure parthenogenetic reproduction.

- Selection of test animals: Females are separated from the offspring 24 hours before the start of the test. The litter produced overnight is used for
the test. The offspring produced overnight are transferred into test water and are used for the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

14°dH = 250 mg CaCO3/L

Test temperature:

20.06-20.28°C

pH:

7.4-8.1

Nominal and measured concentrations:

nominal: 120, 180, 250, 350, 500, 700 and 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 22 ml head-space test tubes with 20 ml volume
- Experimental set-up: 7 test item concentrations + 1 control
- Density of occupation of animals: approx. 2 mL per test organism
- Aeration: none
- Feeding: none

TEST MEDIUM / WATER PARAMETERS
- Stock solution: For the preparation of a stock solution, 2 g/ L of the test item were equilibrated in synthetic fresh water. This solution served as
stock solution.


TEST CONCENTRATIONS
The measured test concentrations after 48 h did not deviate by more than 20% from the freshly prepared test solutions. It was concluded that the test item was present during the whole exposure time. Therefore, the nominal values of the test item concentrations were used for the evaluation of the
test.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

The highest concentration tested in this experiment at which after 48 h exposure no immobilisation (<10%) occurred, was 500 mg/L
The lowest concentration tested in this experiment at which after 48 h exposure 100 % immobilisation occurred, was >1000 mg/L