4-chloro-α,α,α-trifluorotoluene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September - October 1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Please refer to the Executive Summary for details on method.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-chloro-α,α,α-trifluorotoluene

EC Number:

202-681-1

EC Name:

4-chloro-α,α,α-trifluorotoluene

Cas Number:

98-56-6

Molecular formula:

C7H4ClF3

IUPAC Name:

1-chloro-4-(trifluoromethyl)benzene

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Parachlorbenzotrifluorid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 121-148 g
- Housing: plastic cages on sawdust
- Fasting: no food since 16 hs before treatment
- Diet : ALTROMIN 1324 ad libitum
- Water: ad libitum


IN-LIFE DATES: From: 28 September To: 12 October

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

vegetable oil

Remarks:

sesam oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25%

MAXIMUM DOSE VOLUME APPLIED: 10.000 mg/kg

Doses:

4000, 6300, 10000 mg/kg

No. of animals per sex per dose:

10 (males)

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs.

Statistics:

Probit analysis

Results and discussion

Preliminary study:

No

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

5 546 mg/kg bw

Based on:

test mat.

95% CL:

> 4 575 - < 6 724

Mortality:

4000 mg/kg: 1/10 rat
6300 mg/kg: 8/10 rat
10000 mg/kg: 9/10 rat

Clinical signs:

other: convulsions, loss of balance,

Gross pathology:

whitish mucosa in the small intestine

Other findings:

No finding in macroscopic evaluation.

Applicant's summary and conclusion

Interpretation of results:

other: CLP criteria not met

Conclusions:

The test item LD50 is 5546 mg/kg for male rats. The test item results practically non-toxic.

Executive summary:

After a fasting period of 16 days, the test item Parachlorbenzotrifluorid was administered by oral gavage to 10 male Wistar rats for each dose level (4000, 6300 and 10000 mg/kg b/w). The observed mortality 'til day 4 was as follows:

dose level 4000 mg/kg : 1/10 rats died

dose level 6300 mg/kg : 8/10 rats died

dose level 10000 mg/kg: 9/10 rats died

The registered clinical signs were: loss of balance and convulsions.

The LD50 was calculated by Probit analysis and was found to be 5546 mg/kg b/w.