Reaction mass of 3,7-dimethyloct-7-en-1-yl 3-methylbut-2-enoate and 3,7-dimethyloctyl 3-methyl-2-butenoate and citronellyl 3-methylcrotonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

January 1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

After some preliminary observations, the test material was given undiluted, by gavage to groups of five males and five females, in one single dose of 10.0 ml per kg body weight.
After treatment the rats received stock diet and tap water as libitum.
They were observed for signs of intication during 14 day period, after which autopsies were carried out on the survivors.

GLP compliance:

not specified

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

Citronellyl 3-methylcrotonate

EC Number:

244-019-4

EC Name:

Citronellyl 3-methylcrotonate

Cas Number:

20770-40-5

Molecular formula:

C15H26O2

IUPAC Name:

3,7-dimethyloct-6-en-1-yl 3-methylbut-2-enoate

Constituent 2

Reference substance name:

3,7-dimethyloctyl 3-methyl-2-butenoate

EC Number:

275-360-7

EC Name:

3,7-dimethyloctyl 3-methyl-2-butenoate

Cas Number:

71383-07-8

Molecular formula:

C15H28O2

IUPAC Name:

3,7-dimethyloctyl 3-methylbut-2-enoate

Constituent 3

Reference substance name:

3,7-dimethyloct-7-en-1-yl 3-methylbut-2-enoate

Cas Number:

74499-48-2

Molecular formula:

C15H26O2

IUPAC Name:

3,7-dimethyloct-7-en-1-yl 3-methylbut-2-enoate

Test material form:

liquid

Specific details on test material used for the study:

A 50 ml sample of the test material was received from the principal in January 1978. It was a colourless liquid with a pugent odour, designated Sinodor 688 and coded M-76-193.

Test animals

Species:

rat

Strain:

Wistar

Remarks:

Young adult albino rats (wistar-derived)

Sex:

male/female

Details on test animals or test system and environmental conditions:

Young adult albino rats (Wistar-derived) from the Institue's colony were used. The body weights of males varied from 209 to 248g, those of females from 145 to 186g. The rats were housed in groups of five in screen-bottomed stainless steel cages, in a well-ventilated room, maintained at 23-25°C. Before dosing the rats were fasted overnight.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

After some preliminary observations, the test material was given undiluted, by gavage to groups of five males and five females, in one single dose of 10.0 ml per kg body weight.

Doses:

10.0 ml per kg body weight

No. of animals per sex per dose:

5 males and 5 females

Control animals:

no

Details on study design:

After some preliminary observations, the test material was given undiluted, by gavage to groups of five males and five females, in one single dose of 10.0 ml per kg body weight. Due to the small quantity of the test material, no higher doses could be given. After treatment the rats received stock diet and tap water ad libitum. They were observed for signs of intoxication during a 14 day period, after which autopsies were carried out on the survivors.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 mL/kg bw

Based on:

test mat.

Remarks on result:

other: No deaths

Mortality:

No deaths occured

Clinical signs:

other: Within a few hours after dosing the rats showed signs of sluggishness and decreased activity. Later on the rats recovered and looked quite healthy during the remaining part of the observation period. Macroscopic examination of the survivors at autopsy r

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

From these results it appears that the oral LD50 of Sinodor exceeds 10 ml per kg body weight.

Executive summary:

Acute oral toxicity was determined with a method similar to OECD Guideline 401.

At a dose of 10 ml/kg body weight, no deaths occured and clinical signs a few hours after dosing were that the rats showed signs of sluggishness and decreased activity.

Later on the rats recovered and looked quite healthy during the remaining part of the observation period. Macroscopic examination of the survivors at autopsy revealed no treatment related gross alterations.

From these results it appears that the oral LD50 of Sinodor exceeds 10 ml per kg body weight, GHS criteria not met.