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EC number: 949-986-2 | CAS number: 49720-39-0
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-05-14 to 1993-06-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study, the study was available in Japanese, some sections were transalted into English
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes
Test material
- Reference substance name:
- Arginine
- EC Number:
- 200-811-1
- EC Name:
- Arginine
- Cas Number:
- 74-79-3
- Molecular formula:
- C6H14N4O2
- IUPAC Name:
- arginine
- Test material form:
- not specified
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- O2 consumption
- Remarks:
- (BOD)
- Parameter followed for biodegradation estimation:
- TOC removal
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Remarks:
- by HPLC
- Details on study design:
- Cultivating conditions (BOD):
- Concentration of test substance: 100 mg/L
- Concentration of reference substance (aniline): 100 mg/L - Concentration of activated sludge: 30 mg/L
- Temperature: 25 ± 1°C
- Duration: 28 days (1993-05-19 to 1993-06-16)
SAMPLING
- Sampling frequency (BOD): 7th day, 14th day, 21st day, 28th day
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (control blank)
- Other: blank of water, water and test substance
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- BOD
- Value:
- 60
- Sampling time:
- 28 d
- Remarks on result:
- other: mean % Degr
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Remarks:
- HPLC
- Value:
- 100
- Sampling time:
- 28 d
- Remarks on result:
- other: mean % Degr
- Key result
- Parameter:
- % degradation (TOC removal)
- Value:
- 99
- Sampling time:
- 28 d
- Remarks on result:
- other: mean % Degr
- Details on results:
- BOD method: After 28 days cultivation the BOD (mg) of three samples consisting of sludge and test substance was measured. Using BOD and TOC the percentage of biodegradation (%Degr.) of
each sample and the mean percentage of biodegradation (mean %Degr.) was determined.
A comparatively low value of 60% mean biodegradability was observed by the BOD method. The reason for the reduced BOD presumably was that some test substance was incorporated in the body of the microorganisms.
TOC method: After 28 days also the TOC (mgC/l) of the three samples consisting of sludge and test substance was measured. Using TOC and DOC the percentage of biodegradation (%Degr.) of each sample and the mean percentage of biodegradation (mean %Degr.) was determined.
HPLC method: After 28 days also HPLC of the three samples consisting of sludge and test substance was performed. From the residual amount of test substance the percentage of biodegradation (%Degr.) of each sample and the mean percentage of biodegradation (mean % Degr.) was determined.
Additionally, the nitrification of the samples was determined by FIA. The average percentage production of the theoretical amount of converted product (NH4-N) of the three samples
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- L-arginine showed 100% degradation (HPLC) after 28 days in a GLP study according to guideline OECD 301C and therefore can be considered as readily biodegradable
- Executive summary:
L-arginine showed 100% degradation (HPLC) after 28 days in a GLP study according to guideline OECD 301C and therefore can be considered as readily biodegradable
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