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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1993-05-14 to 1993-06-30
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study, the study was available in Japanese, some sections were transalted into English

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Arginine
EC Number:
200-811-1
EC Name:
Arginine
Cas Number:
74-79-3
Molecular formula:
C6H14N4O2
IUPAC Name:
arginine
Test material form:
not specified

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Remarks:
(BOD)
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
by HPLC
Details on study design:
Cultivating conditions (BOD):
- Concentration of test substance: 100 mg/L
- Concentration of reference substance (aniline): 100 mg/L - Concentration of activated sludge: 30 mg/L
- Temperature: 25 ± 1°C
- Duration: 28 days (1993-05-19 to 1993-06-16)

SAMPLING
- Sampling frequency (BOD): 7th day, 14th day, 21st day, 28th day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes (control blank)
- Other: blank of water, water and test substance


Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Remarks:
BOD
Value:
60
Sampling time:
28 d
Remarks on result:
other: mean % Degr
Parameter:
% degradation (test mat. analysis)
Remarks:
HPLC
Value:
100
Sampling time:
28 d
Remarks on result:
other: mean % Degr
Parameter:
% degradation (TOC removal)
Value:
99
Sampling time:
28 d
Remarks on result:
other: mean % Degr
Details on results:
BOD method: After 28 days cultivation the BOD (mg) of three samples consisting of sludge and test substance was measured. Using BOD and TOC the percentage of biodegradation (%Degr.) of
each sample and the mean percentage of biodegradation (mean %Degr.) was determined.
A comparatively low value of 60% mean biodegradability was observed by the BOD method. The reason for the reduced BOD presumably was that some test substance was incorporated in the body of the microorganisms.

TOC method: After 28 days also the TOC (mgC/l) of the three samples consisting of sludge and test substance was measured. Using TOC and DOC the percentage of biodegradation (%Degr.) of each sample and the mean percentage of biodegradation (mean %Degr.) was determined.

HPLC method: After 28 days also HPLC of the three samples consisting of sludge and test substance was performed. From the residual amount of test substance the percentage of biodegradation (%Degr.) of each sample and the mean percentage of biodegradation (mean % Degr.) was determined.

Additionally, the nitrification of the samples was determined by FIA. The average percentage production of the theoretical amount of converted product (NH4-N) of the three samples

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
L-arginine showed 100% degradation (HPLC) after 28 days in a GLP study according to guideline OECD 301C and therefore can be considered as readily biodegradable
Executive summary:

L-arginine showed 100% degradation (HPLC) after 28 days in a GLP study according to guideline OECD 301C and therefore can be considered as readily biodegradable

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