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EC number: 949-957-4
CAS number: -
objective of the present study was to determine the effect of the test
item on growth rate and yield of the unicellular green alga Pseudokirchneriella
study comprised of one control group and one test item group with six
replicates each. A nominal test item concentration of 100 mg/L was
prepared in 100 mL reconstituted water. The initial algae cell density
in each replicate was 10 000 cells per mL. The growth of the algae was
determined over an exposure period of 72 hours and compared with the
growth of the control group.
The test item concentration in the reconstituted water was not
quantified at the start and the end of this study. Because of the low
water solubility (1.663x10 -14 mg/L), the test
item cannot be detected with standard analytical methods and the
development of an analytical method with a sufficiently low detection
and quantification limit is complex.
Due to the absence of any adverse effects at the saturation
concentration, the study was performed without analytical concentration
significant effect on growth rate and yield of the algae were observed
at a nominal concentration of 100 mg/L, i.e. up to the maximum water
following EC values (Growth rate and Yield) were determined:
95% confidence interval
> 1.663x10 -14 mg/L (nominal >100 mg/L)
> 0.067 mg/L (nominal >100 mg/L)
Under the conditions of the present study, an aqueous solution of
nominal 100 mg/L of the test item revealed no toxicity to algae.The
72h EC50 for growth rate and yield were >1.663x10 -14
mg/L (nominal >100 mg/L) and, thus, could not be determined in this
study was performed according to GLP and the methods applied are fully
compliant with OECD TG 201. Under the conditions of
the present study, an aqueous solution of nominal 100 mg/L of the test
item revealed no toxicity to algae.The
72h EC50 for growth rate and yield were > 1.663x10^-14
(nominal >100 mg/L) and, thus, could not be determined in this study.
No adverse effects on algae were observed up to the limit
of water solubility. Thus, the 72 -h EC50 value and 72 -h EC10 value are
greater than 1.663x10 -14 mg/L which corresponds to a nominal
concentration of 100 mg/L.
OECD 201: EC50 (72 h) > 100 mg/L (nominal), EC10 (72 h) >
100 mg/L (1.663x10 -14 mg/L (water solubility))
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