Dipotassium methanedisulphonate

Endpoint summary

Description of key information

Short-term toxicity to invertebrates. Key study: Test method according to OECD 202, GLP study. The 48h-EC50 of the test substance to Daphnia magna was found to be greater than 100.0 mg/L.

Toxicity to aquatic algae and cyanobacteria. Key study. Test method according to OECD 201, GLP study. In the Pseudokirchneriella subpicata green algae toxicity test the 72h-ErC50 of the test substance was determined to be higher than 100.0 mg/L.

Additional information

Short-term toxicity to invertebrates. Key study: An acute aquatic toxicity study with Daphnia magna was conducted on test item according to OECD guideline 202, following GLP. A preliminary range finding study was conducted with test concentrations of 0.0 (control), 0.01, 0.1, 1.0, 10.0 and 100.0 mg/L for a period of 48 h with 60 daphnids, divided into six groups of 10 daphnids per group. Based on no mortalities found in this preliminary study, the main test was restricted to limit study conducted with single test concentration of 100.0 mg/L. Reconstituted water (ISO test water) was used as test medium. In the previous solubility study the test item was found to be soluble in reconstituted water so no vehicle was used for the study. The main test was conducted with test concentrations of 0.0 (control) and 100.0 mg/L for a period of 48 h in static conditions in 4 replicates of 5 daphnids per replicate. The test solutions were not aerated during the exposure. A validated analytical method based on ICP-OES was used to monitor the concentration and stability of the active ingredient in the test solution at 0 and 48 h of the main study. The active ingredient concentration in the test solution was within acceptable limit (> 80% of nominal concentration). All validity criteria were fulfilled. No immobilisation and no abnormal behavioural symptoms were observed over the period of 48 h at the test concentration of 100.0 mg/L as well as in the control group. Thus, EC50 (48 h), NOEC and LOEC to Daphnia magna of the test substance were found to be greater than 100.0 mg/L.

Toxicity to aquatic algae and cyanobacteria. Key study. An Algae Growth Inhibition Test was performed withPseudokirchneriella subpicatagreen algae on the test substance over a period of 72 h in static conditions according to OECD Guideline 201, following GLP. OECD medium was used for algal culturing. In the previous solubility study the test item was found to be soluble in OECD medium so no vehicle was used for the study. A preliminary range finding study, with 2 replicates for test item and 3 replicates for control, was conducted with the test concentrations of 0.0 (control), 0.01, 0.1, 1.0, 10.0 and 100.0 mg/L, resulting in percent inhibitions of growth rate of 0.00, -0.15, 0.15, 0.15 and 0.15, respectively. Based on these results, the test concentrations selected for the main study were 0.0 (control) and 100.0 mg/L. Six replicates for the control group and the treatment group were used. 1.4 mL of algal culture was inoculated into the test vessel for each replicate to obtain an initial mean cell concentration of 6234 cells/mL. A validated analytical method based on ICP-OES was used to monitor the concentration and stability of the main active ingredients in the test solution at 0 and 72 h. The active ingredient concentration in the test media was within acceptable limit (> 80% of nominal concentration). All validity criteria were fulfilled. There was no significant alteration observed in algal growth (biomass), yield and growth rate of the test item exposed group as compared to control group. The test substance does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the concentration of 100.0 mg/L. The ErC50, NOEC and LOEC value was greater than 100.0 mg/L.