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EC number: 947-397-5
CAS number: -
The toxic effects of the test substance on the oxygen consumption rate of aerobic micro-organisms (activated sludge) after short-term exposure of 30 min was determined according to the OECD guideline No. 209 (1993). The EC50 was determined to be greater than 1000 mg/L, the EC20 was determined to be 650 mg/l. All values based on nominal test concentrations.
The objective of this key study
according to OECD guideline No. 209 (1993) was to determine the toxic
effects of the test substance on the oxygen consumption rate of aerobic microorganisms
(activated sludge) after short-term exposure of 30 min, compared with
the blank control. The test was performed in nominal concentrations of
100, 500 and 1000 mg/L. The inoculum was activated sludge from
laboratory waste water treatment plant of the company BASF (Germany) in
a concentration of 1 g/L. The EC50 value of for the reference substance
3,5-dichlorophenol was determined to be ca. 20 mg/L, which was within
the acceptable limits, (5.0 to 30 mg/L) as specified in OECD 209
Guideline. All validation criteria were fulfilled. The EC50 was
determined to be greater than 1000 mg/L.
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