2-methyl-3-butenenitrile

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Few data are missing.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1970

Report date:

1970

Materials and methods

Principles of method if other than guideline:

For each exposure, six ChR-CD male rats were exposed to the test substance an a 16-liter bell jar for four hours. The test substance was administered at a uniform rate into a jeated (140-165 °C ) stainless steel T-tube by a syringue drive and vaporized under prepurified nitrogen. The test
substance vapors were mixed with oxygen and carried into the bell jar; houseline air was used as diluent to give the desired atmospheric concentration.
For analysis, gas samples were taken periodically from the chamber exit and analyzed by a gaz chromatographic method.
Gross and histopathologic examination were performed on two rats each 1, 2, 7 and 14 days post-exposure. The other survivors were sacrified 14
days post-exposure.

GLP compliance:

not specified

Remarks:

The study was performed before GLP statment

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methyl-3-butenenitrile

EC Number:

240-596-1

EC Name:

2-methyl-3-butenenitrile

Cas Number:

16529-56-9

Molecular formula:

C5H7N

IUPAC Name:

2-methylbut-3-enenitrile

Details on test material:

- Name of test material (as cited in study report): 2-methyl-3-butenenitrile (2M3BN)
- Substance type: 2-methyl-3-butenenitrile (2M3BN)
- Physical state: No data
- Analytical purity: approximately 100 %
- Impurities (identity and concentrations): No data
- Composition of test material, percentage of components: No data
- Purity test date: No data
- Lot/batch No.: No data
- Expiration date of the lot/batch: No data
- Stability under test conditions: No data
- Storage condition of test material: No data
- Other: No data

Test animals

Species:

rat

Strain:

other: ChR-CD

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: No data
- Age at study initiation: No data
- Weight at study initiation: 250-289 grams
- Fasting period before study: No data
- Housing: in cages
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: No data


ENVIRONMENTAL CONDITIONS
No data


IN-LIFE DATES: No data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: houseline air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
No data


TEST ATMOSPHERE
- Brief description of analytical method used: No data
- Samples taken from breathing zone: yes


VEHICLE
- Composition of vehicle (if applicable): houseline air was used as diluent to give the desired atmospheric concentration
- Concentration of test material in vehicle (if applicable): houseline air was used as diluent to give the desired atmospheric concentration
- Justification of choice of vehicle: the test substance was mixed with oxygen and houseline air was used as diluent to give the desired atmospheric concentration
- Lot/batch no. (if required): No data
- Purity: No data


TEST ATMOSPHERE (if not tabulated)
No data


CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration: No data

Analytical verification of test atmosphere concentrations:

yes

Remarks:

analysed periby a gaz chromatographic method

Duration of exposure:

4 h

Concentrations:

815; 1060; 1540; 1812; 2416; 2870; 3200 and 3835 ppm (volume to volume basis)

No. of animals per sex per dose:

6

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Gross and histopathologic examination were performed on two rats each 1, 2, 7 and 14 days post-exposure.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Statistics:

No data

Results and discussion

Preliminary study:

No preliminary study

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 3 000 ppm

95% CL:

>= 2 760 - <= 3 261

Exp. duration:

4 h

Mortality:

The mortality was 0/6; 0/6; 0/6 ; 1/6; 1/6; 3/6 and 5/6 respectively for 815; 1060; 1540; 1812; 2416; 2870; 3200 and 3835 ppm.
First death occured within 3.5 hours.
Death overnight.

Clinical signs:

other: During exposure, the symptoms were the following: - Lethal concentrations: Irregular respiration, incoordination, lacrimation, salivation, inflamed eyes, red discharge from eyes, hyperemia, tremors, unresponsiveness to sound, pale ears - Non lethal concen

Body weight:

Initial weight loss followed by normal weight gain was observed at lethal and non-lethal concentrations.

Gross pathology:

Gross and histopathologic examinations of the rats showed no anatomical evidence of primary injury.

Other findings:

No data

Any other information on results incl. tables

No additional data

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Under the test conditions of this study, LC 50/rat (male)/4 h = 3000 ppm (9.95 mg/L). Therefore, 2 -methyl-3-butenenitrile is classified
in category III (H331: Toxic if inhaled) according to the CLP regulation (1272/2008) and Harmful (Xn, R20) according to the Directive 67/548/EEC.

Executive summary:

In an acute toxicity study, 2 -methyl-3-butenenitrile was administered to six ChR-CD male rats by inhalation way at 3000 ppm for four hours. Gross and histopathologic examinations were performed on two rats each 1, 2, 7 and 14 days post-exposure.

The other survivors were sacrified 14 days post-exposure.

Clinical signs observed in the rats indicated an effect on the central nervous system. First death occured within 3.5 hours.

Under the test conditions of this study, LC 50/rat (male)/4 h = 3000 ppm (9.95 mg/L). Therefore, 2 -methyl-3-butenenitrile was considered in category III (H331: Toxic if inhaled) according to the CLP regulation and as harmful if inhaled (Xn, R20) according to the Directive 67/548/EEC.