Benzene-1,4-diammonium sulphate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of read across chemicals

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention in below principle

Principles of method if other than guideline:

WoE report is prepared based on toxicity to microorganisms study including WoE 2, 3 and 4.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material (IUPAC name): Benzene-1,4-diammonium sulphate- Molecular formula: C6H8N2.H2O4S- Molecular weight: 206.221 g/mol- Smiles notation: S(=O)(=O)(O)O.c1(ccc(cc1)N)N- InChI: 1S/C6H8N2.H2O4S/c7-5-1-2-6(8)4-3-5;1-5(2,3)4/h1-4H,7-8H2;(H2,1,2,3,4)- Substance type: Organic - Physical state: Solid

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

1, 2nd study DMSO

Details on test solutions:

1,2: - Method: Stock solutions of each were formulated by dissolving the chemical at concentrations of 5, 10, 50, or 100 ug/ml in dimethyl sulfoxide (DMSO) or sterile distilled water.

Test organisms (species):

other: 1, 2nd study: Tetrahymena pyriformis. 3rd study Ruminococcus albus and Butyrivibrio fibrisolvens

Details on inoculum:

Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime.

Test type:

static

Water media type:

freshwater

Total exposure duration:

60 h

Test temperature:

1, 2: 28 °C

Details on test conditions:

Details on test conditionsTEST SYSTEM- Test vessel: Erlenmeyer flask- Material, size, headspace, fill volume:250 ml Erlenmeyer flask containing 50 ml of test solution- No. of vessels per concentration (replicates): 2- No. of vessels per control (replicates): 3 EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured.

Reference substance (positive control):

yes

Remarks:

1, 2 study: 2-ketoalkane

Key result

Duration:

60 h

Dose descriptor:

other: IGC50

Effect conc.:

10.38 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 95% CI was 4.52 – 13.91 mg/l

Duration:

60 h

Dose descriptor:

other: IGC50

Effect conc.:

7.35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 95% CI was 5.95 – 8.98 mg/l

Dose descriptor:

other: IGC

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Growth inhibition were observed in Ruminococcus albus

Reported statistics and error estimates:

1,2: Probit analysis

Validity criteria fulfilled:

not specified

Conclusions:

1. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.2. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l.3. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l. Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.

Executive summary:

Toxicity of Benzene-1,4-diammonium sulphate (CAS No. 16245 -77 -5) on the growth and other biological activity of microorganisms is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

In the first study toxicity was measured.

Study was conducted to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.

Similarly aim of this study was to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l with 95% CI was 5.95 – 8.98 mg/l.

In third study toxicity were observed at 10 mg/l. Aim of this study was to determine the toxicity of test chemical on the growth of microorganisms. Test conducted on two different organisms Ruminococcus albus and Butyrivibrio fibrisolvens. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l.

Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.

Description of key information

1. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.

2. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l.

3. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l.

Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

10.38 mg/L

Additional information

Toxicity of Benzene-1,4-diammonium sulphate (CAS No. 16245 -77 -5) on the growth and other biological activity of microorganisms is predicted on the basis of it structurally and functionally similar read across chemicals. The studies are as mentioned below:

 

In the first study toxicity was measured. Study was conducted to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 10.38 mg/l.

 

First study was supported by the second experimental study from peer reviewed journal. Aim of this study was to investigate the effect of test chemical on the growth of ciliate Tetrahymena pyriformis. Test conducted under the static system for 60 hrs. Chemical analytically monetarized by using HPLC. DMSO used as a solvent. Axenic cultures of T. pyriformis reared in a semidefined protease peptone medium were grown at 28°C under a static regime. 250 ml Erlenmeyer flask containing 50 ml of test solution. Duplicate assembly were arrange for the test. Tetrahymena pyriformis was used as the bioassay system. Population growth and density being selected on which toxicity were measured. Based on the population growth inhibition of Tetrahymena pyriformis by the test chemical after 60 hrs, the IGC50 was observed at 7.35 mg/l with 95% CI was 5.95 – 8.98 mg/l.

 

In third study toxicity were observed at 10 mg/l. Aim of this study was to determine the toxicity of test chemical on the growth of microorganisms. Test conducted on two different organisms Ruminococcus albus and Butyrivibrio fibrisolvens. As the two different species Ruminococcus albus and Butyrivibrio fibrisolvens exposed with the test chemical, effect on the growth of organisms vary. The IGC for Ruminococcus albus was determine to be 10 mg/l and on the other species it was observed that the growth inhibited at 100 mg/l.

 

Thus based on the overall studies for Benzene-1,4-diammonium sulphate(CAS No. 16245 -77 -5), toxicity value ranges from 10 mg/l to 10.38 mg/l.