Cyclohexane, oxidized, aq. ext., sodium salt

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German Industrial Standard DIN 38412 part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

sodium carboxylate solution

IUPAC Name:

sodium carboxylate solution

Details on test material:

- name: Sodium carboxylate solution
- purity: not specified

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

- Test fish: Leuciscus idus (golden variety)
- Supplier: Fischzucht Paul Eggers, 2354 Hohenweststedt, Germany
- Body length: 5.8 cm (range 5.1 - 6.4 cm)
- Body weight: 2.4 g (range 1.8 - 3.3 g)
- Loading rate (g fish/l test water): 2.4
- Medical treatment: twice with 0.05 mg/l malachite green chloride, once with 10 mg/l tetracycline hydrochloride

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 - 21 °C

pH:

7.6 - 10.0

Dissolved oxygen:

7.5 - 8.7 mg/l

Details on test conditions:

- Dilution water source: reconstituted freshwater according to DIN 38412, Part II, 1982

Dilution water chemistry
- Total hardness: 2.5 mmol/l
- Acid capacity: 0.8 mmol/l
- Ratio Ca/Mg ions: 4:1
- Ratio Na/K ions: 10:1
- pH: about 7.7
- The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria. Nominal test concentrations: 5000 and 10000 mg/l. In addition, a control was tested in parallel.
- Test vessels: All glass aquaria (30 x 22 x 24 cm)
- Volume of water: 10 l
- Continuous aeration with oil-free air, slight
- Photoperiod: 16 : 8 hours day-night regime
- One test vessel per treatment
- No of animals per test concentration and control: 10
- Duration of adaption: about 2 weeks
- Mortality during the adaption period: 0 %
- Withdrawal of food: 1 day before and during exposure.
- observations: mortality and symptoms after 0, 4, 24, 48, 72, and 96 h
- measurements: pH and oxygen content after 0, 24, 48, 72 and 96 h
- Statistical methods: The median lethal concentration (LC50) was calculated using Probit Analysis (Finney D.J., Probit Analysis, Cambr. Univ. Press, 3rd Edition, 1971)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

RS-Freetext:
No analytical dose-verification of the test item was carried out.

LC 50 (48h): > 10000 mg/l
LC 50 (72h): > 10000 mg/l
LC 50 (96h): > 10000 mg/l

Table showing cumulative mortality:
Cumulative mortality

Nom. conc. (mg/l) 1h 4h 24h 48h 72h 96h
Control 0 0 0 0 0 0
5000 0 0 0 0 0 0
10000 0 0 0 0 0 0

No symptoms observed

Reference substances (if used):
48 h-LC50 of the positive control Chloroacetamide: about 32 mg/l (corresponds to normal sensitivity).


Behavior of the test item during the test:

No remarkable observations.