Disodium 7-[[4,6-bis[(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl]amino]-4-hydroxy-3-[[4-[(4-sulphonatophenyl)azo]phenyl]azo]naphthalene-2-sulphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

May from 13th to 18th, 1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

Internal method JIS - 0102, 1974

GLP compliance:

no

Details on test organisms:

TEST ORGANISM
- Common name: orange-red killifish.
- Order: cyprinodontiformes.
- Average weight: 0.27 g
- Average length: 3.1 cm
- Selection: fish passed mercuric-chloride inspection.

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

48 h

Test temperature:

25 ± 1 °C

pH:

7.7 (end of test)

Dissolved oxygen:

4.8 (end of test)

Nominal and measured concentrations:

1000 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium made of glass.
- Size: 4 litres.
- No. of organisms per vessel: 10

TEST MEDIUM
- Source of dilution water: water aerated enough was used as dilution water.

Duration:

48 h

Dose descriptor:

other: TLm

Effect conc.:

> 1 000 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The sample resulted to be water soluble.

Validity criteria fulfilled:

not specified

Conclusions:

TLm (48 h) > 1000 ppm

Executive summary:

The short-term toxic potential of the test substance to orange-red killifish was investigated according to a specific national testing guideline: JIS - 0102 - 1974. 10 fish were exposed to a test concentration of 1000 ppm at a test temperature of 25 ± 1 °C. The duration of the test was 48 h. The fish which passed mercuric chloride inspection were used. Well-water aerated enough was used as the dilution water. Survival rates in percentage were determined at 2 h, 24 h and 48 h.

The Median Tolerance Limit (48 h) resulted to be greater than 1000 ppm, therefore, the test substance can be considered as non-toxic to fish.

 

Conclusion

TLm (48 h) > 1000 ppm

Description of key information

Not harmful/toxic for fish after short-term exposures.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L

Additional information

The short-term toxic potential of the test substance to orange-red killifish was investigated according to a specific testing method: JIS - 0102 - 1974. 10 fish (which passed mercuric chloride inspection) were exposed to a test concentration of 1000 ppm over a period of 48 hours. Survival rates in percentage were determined at 2 h, 24 h and 48 h. The Median Tolerance Limit (48 h) resulted to be greater than 1000 ppm, therefore, the test substance has been judged as non-toxic to fish.

The study outcomes are confirmed by further information available on Direct Red 253 (on substance as itself and on the substance salificated by both sodium and triethanolammonium salts (50:50)). Unfortunately, only a short sheet of ecotoxicological data is currently available, thus reliability cannot be assigned; many details are missing on test procedures and on the study results. In any case, the LC50 values agree with that recorded in the abovementioned experiment.