Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 211-617-1 | CAS number: 674-82-8
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well performed and reported study similar to current OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Deviations:
- no
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- But-3-en-3-olide
- EC Number:
- 211-617-1
- EC Name:
- But-3-en-3-olide
- Cas Number:
- 674-82-8
- Molecular formula:
- C4H4O2
- IUPAC Name:
- 4-methylideneoxetan-2-one
- Reference substance name:
- Diketene
- IUPAC Name:
- Diketene
- Details on test material:
- no data
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Origine: IFFA CREDO, Apres une periode d`adaption d`une semaine a l`animalerie, les animeaux sont stabules par groupe de 5.
soit dans les cages en Makrolon comportant une litiere de sciure de bois sterilisee et depoussieree, en dehors des periodes d`inhalation
soit dans les cages grillagees en acier inoxydable situees dans un meme plan pendant les seances d`inhalation
Aliment: Souriffarat (en dehors de la periode d`inhalation)
Abreuvement: Eau ad libitum (en dehors de la periode d`inhalation)
Environment (Animalerie): Renouvellement d`air: 8 par heure, Temperature: 22 +- 1,5 °C, Hygrometrie: 55 % +- 15 %, Eclairment: 12/24 ( par minuterie)
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- Les animeaux sont exposes au produit aerosolise a une concentration connue dans l`air et pendant un temps determine. On utilise la technologie avec un perfuser pour doser le diketene, un debimetre a flotteur pour determine la quantite d`air correspondant et un seche-cheveaux pour evaporer diketene condense.
- Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 4 h
- Concentrations:
- 0.452 g/ccm, 0.677 g/ccm, 0.787 g(ccm, 0.847 g/ccm, 1.142 g/ccm, 1,700 g/ccm
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- 14 d observation period
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 940 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 1 008 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 820 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- at 0.452 g/ccm, 0.677 g/ccm, 0.787 g(ccm, 0.847 g/ccm, 1.142 g/ccm, 1,700 g/ccm: 0 %, 0 %, 40 %, 60 %, 100 %, 100 % male dead after 14 d and 0 %, 0 %, 20 %, 0 %, 60 %, 100 % female dead after 14 d
- Clinical signs:
- other: Detresse respiratoire, Fermeture de la fente palpebrale, Dyspnee - larmoiements - rhinorrhee et leger epistaxis
- Body weight:
- 150 - 200g
- Gross pathology:
- Il apparait qu`un oedeme aigu hemorragique du poumon soit responsable de la mort des animeaux. La frquence de cette alteration est fonction de la dose administree. Li s`y associe une congestion hepatique, ceci est classique dans l`oedeme aigu pulmonaire qui entaine une defaillance du coeur droit.
Applicant's summary and conclusion
- Interpretation of results:
- Toxicity Category II
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The LC50 (4h) of Diketene for rats was derived at 940 ppm corresponding to 0.94 mg/L , for male rats at 820 ppm corresponding to 0.82 mg/L and for female rats at 1008 mg/L corresponding to 1.01 mg/L
- Executive summary:
The LC50 of Diketene was determined in an acute inhalation study. Groups of male and female rats were exposed to atmospheres of 0.452 g/ccm, 0.677 g/ccm, 0.787 g(ccm, 0.847 g/ccm, 1.142 g/ccm, 1,700 g/ccm for 4 hours. The LC50 (4h) of Diketene for rats was derived at 940 ppm corresponding to 0.94 mg/L , for male rats at 820 ppm corresponding to 0.82 mg/L and for female rats at 1008 mg/L corresponding to 1.01 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
