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EC number: 943-080-0
CAS number: -
Main results from experiment I and II (Mean values of both
Relative Total Growth [%]
Mutants per 106Cells
Solvent Control of Test Item
Solvent Control of Positive Control
Test Item 5.00 µL/mL
Test Item 0.63 µL/mL
Test Item 0.31 µL/mL
Test Item 0.16 µL/mL
Test Item 0.08 µL/mL
Test Item 0.04 µL/mL
Test Item 0.02 µL/mL
- Cultures could not be evaluated for mutagenicity because of
* Cultures were not continued since only 4 analysable
concentrations are required by the guideline.
Note: The threshold (number of mutant colonies per 106cells
of the respective solvent control plus 126) was 255 (+S9) and 253 (-S9)
in Experiment I as well as 242 (+S9) and 208 (-S9) in Experiment II.
This study was performed to investigate the potential of the
substance to induce mutations at thethymidine kinase locus(Tk1)
on chromosome 11 and/or structural chromosomal aberrations in mouse
The assay was performed in a pre-test and in two independent main
experiments (experiment I and II) whereby the first experiment I was
invalid and had to be repeated. The results of the invalid experiment
will not be included in this report but will be archived with the raw
data. Therefore, in total one pre-test and three experiments were
The pre-test was done to detect a potential cytotoxic effect of
the test item. Based on the results of this test the concentrations for
the main experiments were determined.
Experiment I was performed with and without metabolic activation
(liver enzyme S9 fraction / “liver S9 mix from male rats, treated with
Aroclor 1254”) and a treatment period of 4 h. Experiment II was
performed with a treatment period of 24 hours in the absence of
metabolic activation and 4 hours in the presence of metabolic activation.
The highest nominal concentration that was used in the experiment
was 5 µL/mL.
Not all tested concentrations could be evaluated for mutagenicity.
Some of them induced a cytotoxic reaction and had to be excluded from
the evaluation of the mutagenicity. However in all analysable
concentrations no substantial and reproducible dose dependent increase
in mutant colony numbers was observed in both experiments. No relevant
shift of the ratio of small versus large colonies was observed up to the
maximal concentration of the test item.
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