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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
April 10 - 24, 1980
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1980
Report date:
1980

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium (xylenes and 4-ethylbenzene) sulphonate
EC Number:
943-024-5
Molecular formula:
C8H13NO3S
IUPAC Name:
Ammonium (xylenes and 4-ethylbenzene) sulphonate

Test animals

Species:
rat
Strain:
other: albino, COX-SD
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS- Source: Lab Supply Company- Age at study initiation: no data- Weight at study initiation: 217 - 240 grams- Fasting period before study: not fasted- Housing:individually in metal, wire-bottomed cages elevated above the droppings- Diet (e.g. ad libitum): ad libitum- Water (e.g. ad libitum): ad libitum- Acclimation period:no dataENVIRONMENTAL CONDITIONS- Temperature (°C): no data- Humidity (%): no data- Air changes (per hr): no data - Photoperiod (hrs dark / hrs light): no data IN-LIFE DATES: From: April 10, 1980 To: April 24, 1980

Administration / exposure

Route of administration:
inhalation: aerosol
Vehicle:
other: unchanged (no vehicle)
No. of animals per sex per dose:
5 males and 5 females; single dose
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days - Frequency of observations and weighing: at regular intervals during the first day and once daily thereafter- Necropsy of survivors performed: yes- Other examinations performed: clinical signs, body weight,histopathology, other: behavior and stool- Upon removal from the chamber, the animals were cleaned with lukewarm water to remove any test material having accumulated on their coats and dried with toweling. Animals were then placed in individual cages.- Duration of observation period following administration: 14 days (or other?)- Frequency of observations and weighing:- Necropsy of survivors performed: yes/no- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:

Results and discussion

Preliminary study:
none reported
Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 6.41 mg/L air (nominal)
Based on:
test mat.
Mortality:
No deaths
Clinical signs:
other: One animal displayed a pronounced case of soft stool on Day 6 an returned to normal in two days. Otherwise no gross signs of test compound induced adverse effects were observed in the remaining nine animals
Body weight:
Slight weight gains in two animals at 7 and 14 days. The body weights for the remaining 8 animals showed gains within limits of expectation.
Gross pathology:
Five animals showed slight mottling or slight to moderate congestion of the lungs. The remaining five showed tissues to be not remarkable

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
LC50 > 6.41 mg/L based on test material
Executive summary:

The acute inhalation toxicity of Ammonium (xylenes and 4-ethylbenzene) sulfonates was assessed following official method OECD 403, Acute Inhalation Toxicity. One animal displayed a pronounced case of soft stool on Day 6 an returned to normal in two days. No gross signs of test compound induced adverse effects were observed in the remaining nine animals, there was no mortality and half of the exposed animals showed only slight to moderate congestion of the lungs.
Slight weight gains in two animals at 7 and 14 days. The body weights for the remaining 8 animals showed gains within  limits of expectation. Five animals showed slight mottling or slight to moderate congestion of the lungs. The remaining animals did not showed remarkable effects.