2-chloropyridine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation.

Data source

Reference

Reference Type:

secondary source

Title:

Unnamed

Year:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methylpyridine

EC Number:

203-636-9

EC Name:

3-methylpyridine

Cas Number:

108-99-6

IUPAC Name:

3-methylpyridine

Details on test material:

- Name of test material (as cited in study report): 3-Methylpyridine

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
- Melting point: -18.1ºC
- Boiling point: 143-144ºC
- Vapour pressure: 6.05 mm Hg at 25ºC
- Water solubility (under test conditions):
- Henry's law constant: 7.73 x 10E-6 atm-cu m/mol at 25ºC
- log Pow: 1.20
- pKa: 5.63 at 25ºC

Reference: HSDB® is provided quarterly by US National Library of Medicine and was last updated December 18, 2009

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Solutions of the substance were prepared by adding an aliquot of a 10 g test substance/L of stock solution to each test chamber. Test solutions were prepared fresh each day of the test.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Common name: Zebra fish

Mean body weight and mean length of 30 fish used in testing were 0.42 g and 3.3 cm, respectively.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

250 ± 50 mg/L as CaCO3

Test temperature:

22 ± 2ºC

pH:

7.9 ± 0.3

Dissolved oxygen:

>= 74 % saturation at 20ºC

Nominal and measured concentrations:

Nominal concentrations: 0, 100, 180, 320, 560 and 1000 mg/L

Details on test conditions:

Test vessels were 8.3-L all-glass aquarium filled with 5 L of dilution water. They were fitted with aeration tubes, covered with a glass pane during the test and held in a water-bath at 22 ± 2ºC under a 16 hour light/8 hour dark photoperiod.

The pH, dissolved oxygen concentration and temperature of the test media were measured at the beginning and the end of the test. Fish were inspected at the beginning of the test and after approximately 2, 4, 24, 48, 72, and 96 hours for lethality and any behavior different from the control group.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 560 - < 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 3

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

560 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

No statistics applied to data.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified