2-chloropyridine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study without detailed documentation.

Data source

Reference

Reference Type:

review article or handbook

Title:

Biodegradation and Bioconcentration of Existing Chemical Substances under the Chemical Substances Control Law

Author:

National Institute of Technology and Evaluation

Year:

1992

Bibliographic source:

Official Bulletin of Economy, Trade and Industry (Former title: The Official Bulletin of the Ministry of International Trade and Industry (published before Jan.6,2001))

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-chloropyridine

EC Number:

203-646-3

EC Name:

2-chloropyridine

Cas Number:

109-09-1

Molecular formula:

C5H4ClN

IUPAC Name:

2-chloropyridine

Details on test material:

- Name of test material (as cited in study report): 2-chloropyridine

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Sludge sampling were made at the following 10 places in Japan:
- Fukogawa city sewage plant (Sapporo-shi Hokkaiso)
- Fukashiba industry sewage plant (Kashima-gun Ibaragi)
- Nakahama city sewage plant (Osaka-shi Osaka)
- Ochiai city sewage plant (Shinjuku-ku Tokyo)
- Kitakami river (Ishinomaki-shi Miyagi)
- Shinano river (Nishikanbara-gun Niigata)
- Yoshino river (Tokushima-shi Tokushima)
- Lake Biwa (Otsu-shi Shiga)
- Hiroshima bay (Hiroshima-shi Hiroshima)
- Dookai bay (Kitakyushu-shi Fukuoka)

Collected March, June, September, and December in every year.

Sampling method - city sewage: returned sludge of sewage plants were collected
Sampling method - rivers, lakes and sea: surface water and surface soil which is in contact with atmosphere were collected

Mixing of fresh and old activated sludge: 5 L of the filtrate of the supernatant of an activated sludge in the present use was mixed with 500 mL of the filtrate of the supernatant of a newly collected sludge respectively and the mixture was cultured at pH 7.0 ± 1.0 under aeration.

Activity of the sludge was inspected to use reference substance. The relation between new and old activated sludge was noted.

Duration of test (contact time):

14 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

TOC removal

Details on study design:

Preparation of test solutions:
a) water + test substance
b) sludge and test substance
c) sludge and reference substance
d) sludge (control)

Test solutions were stirred by a magnetic stirrer.

Cultivation temperature 25 ± 1ºC
Cultivation duration 14 days

After 14 days of cultivation, total organic carbon and the test substance in the test solutions were determined. The pH of the test solutions of (water + test substance) and (sludge + test substance) were measured.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (test mat. analysis)

Value:

0.7

Sampling time:

14 d

% Degradation 2

Parameter:

% degradation (O2 consumption)

Value:

0.5

Sampling time:

14 d

BOD5 / COD results

Results with reference substance:

Not reported.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Interpretation of results:

under test conditions no biodegradation observed