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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
28 may to 16 July 1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well documented guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chloro-4,6-dimethoxy-1,3,5-triazine
EC Number:
221-541-0
EC Name:
2-chloro-4,6-dimethoxy-1,3,5-triazine
Cas Number:
3140-73-6
Molecular formula:
C5H6ClN3O2
IUPAC Name:
2-chloro-4,6-dimethoxy-1,3,5-triazine

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
arachis oil
Doses:
Range finding:
5000 mg/kg, 2000 mg/kg, 1000 mg/kg, 500 mg/kg, 200 mg/kg,
Main study
707 mg/kg, 1000 mg/kg, 1400 mg/kg,
No. of animals per sex per dose:
Main study 5 per sex

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
870 mg/kg bw
Based on:
test mat.
95% CL:
> 812 - < 933

Any other information on results incl. tables

Dose level mg/kg

Sex

Number of Animals treated

Deaths During Day of Dosing (hour)

Deaths During Day of Dosing (days)

Deaths

%

1/2

1

2

4

1

2

3

4

5

6

7

8-14

707

Male

female

5

5

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

1000

Male

female

5

5

0

0

0

0

0

0

0

0

4

5

0

-

0

-

0

-

0

-

0

-

0

-

0

-

0

-

0

-

1414

Male

female

5

5

0

0

2

2

0

1

0

1

3

1

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Applicant's summary and conclusion

Interpretation of results:
harmful
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
2-Chloro-4,6-dimethoxy-1,3,5-Triazin has a LD50 of 870 mg/kg when administred orally. Hence it has to be classified as harmful.
Executive summary:

A study with Spague-Dawley rats was performed according to OECD 401. After a range finding study the doses 707 mg/kg 1000 mg/kg and 1414 mg/kg were given by gavage. Deaths and clinical signs were observed.

The acute oral median lethal dose LD50 and 95% confidence limit of the test material were calculated by the method of Thompson W R to be: 870 (812 - 913) mg/kg bodyweight.