2-chloro-4,6-dimethoxy-1,3,5-triazine

Endpoint summary

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 may to 16 July 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well documented guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Route of administration:

oral: gavage

Vehicle:

arachis oil

Doses:

Range finding:
5000 mg/kg, 2000 mg/kg, 1000 mg/kg, 500 mg/kg, 200 mg/kg,
Main study
707 mg/kg, 1000 mg/kg, 1400 mg/kg,

No. of animals per sex per dose:

Main study 5 per sex

Sex:

male/female

Dose descriptor:

LD50

Effect level:

870 mg/kg bw

Based on:

test mat.

95% CL:

> 812 - < 933

Dose level mg/kg	Sex	Number of Animals treated	Deaths During Day of Dosing (hour)				Deaths During Day of Dosing (days)								Deaths	%
			1/2	1	2	4	1	2	3	4	5	6	7	8-14
707	Male female	5 5	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0	0 0
1000	Male female	5 5	0 0	0 0	0 0	0 0	4 5	0 -	0 -	0 -	0 -	0 -	0 -	0 -	0 -	0 -
1414	Male female	5 5	0 0	2 2	0 1	0 1	3 1	- -	- -	- -	- -	- -	- -	- -	- -	- -

Interpretation of results:

harmful

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

2-Chloro-4,6-dimethoxy-1,3,5-Triazin has a LD50 of 870 mg/kg when administred orally. Hence it has to be classified as harmful.

Executive summary:

A study with Spague-Dawley rats was performed according to OECD 401. After a range finding study the doses 707 mg/kg 1000 mg/kg and 1414 mg/kg were given by gavage. Deaths and clinical signs were observed.

The acute oral median lethal dose LD50 and 95% confidence limit of the test material were calculated by the method of Thompson W R to be: 870 (812 - 913) mg/kg bodyweight.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

870 mg/kg bw

Quality of whole database:

sufficient

Acute toxicity: via inhalation route

Endpoint conclusion

Endpoint conclusion:

no study available

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-01-16 to 1995-02-15

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: no guideline followed but sufficientlydocumented

Qualifier:

no guideline followed

Principles of method if other than guideline:

Substance was applied to clipped skin of a rabbit
Mortality and clinical abnormalities were observed

GLP compliance:

no

Test type:

standard acute method

Species:

rabbit

Strain:

New Zealand White

Sex:

not specified

Type of coverage:

occlusive

Vehicle:

water

Duration of exposure:

24h

Doses:

1000mg/kg bw

No. of animals per sex per dose:

3 animals per dose

Sex:

not specified

Dose descriptor:

LD0

Effect level:

> 1 000 mg/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

other: Substance is considered corrosive to rabbit skin.

Study abnormalities	Animals affected No observed / No Dosed	Day(s) of Occurrence
Mortality	0/3	-
Well-defined erythema	3/3	1-12
Very slight erythema	2/3	13-14
Blanching – mild	2/3	2-8
Blanching - moderate	2/3	5
Blanching – focal/pinpoint	2/3	3,4,8,9
Eschar – mild	2/3	4-14
Eschar - focal/pinpoint	2/3	2,3,10-14
Desquamation	3/3	6-14
Severe edema	3/3	1-4
Moderate edema	3/3	3-7
Slight edema	3/3	5-9
Very slight edema	3/3	9-14
Fecal stain	1/3	1,2
Few feces	3/3	3-5
Low food consumption	2/3	3,4
Dehydration	1/3	4-6
Ancillary irritation on the trunk outside the test site	1/3	1-7

Interpretation of results:

relatively harmless

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

2-Chloro-4,6-Dimethoxy-1,3,5-Triazine does not result in mortality of Rabbits with 1000mg/kg bw. no classification for dermal toxicity is necessarry

Executive summary:

Based on the result of this study, the acute dermal Median Lethal Dose (MLD) of 08699 (equivalent to 2-Chloro-4,6-Dimethoxy-1,3,5-Triazine) was estimated to be greater than 1000 mg/kg in the rabbit. The calculated primary irritation indes was 6.33 which classifies this dose level as a severe irritant to the dermal tissue of the rabbit.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

sufficient

Additional information

Justification for selection of acute toxicity – oral endpoint
guideline study with sufficient documentation

Justification for selection of acute toxicity – dermal endpoint
no guideline followed but sufficiently documented and only study available

Justification for classification or non-classification

Classification acut oral toxicity 4 because of LD50 oral is found to be 870 mg/kg bw in a guideline study.

No classification for acute dermal tox because in a non guideline study there were no deaths oserved up to 1000mg/kg bw.